在实验室实践中如何处理脑脊液淀粉样蛋白β（1-42）的吸附？通过使用 Aβ/Aβ 比值来识别有问题的处理方法并解决该问题。
How to handle adsorption of cerebrospinal fluid amyloid β (1-42) in laboratory practice? Identifying problematic handlings and resolving the issue by use of the Aβ/Aβ ratio.
机构信息
Neurochemistry Laboratory, Department of Clinical Chemistry, Neuroscience Campus Amsterdam, VU University Medical Center, Amsterdam, The Netherlands; Department of Neurology, Alzheimer Center, Neuroscience Campus Amsterdam, VU University Medical Center, Amsterdam, The Netherlands.
Neurochemistry Laboratory, Department of Clinical Chemistry, Neuroscience Campus Amsterdam, VU University Medical Center, Amsterdam, The Netherlands.
出版信息
Alzheimers Dement. 2017 Aug;13(8):885-892. doi: 10.1016/j.jalz.2017.01.010. Epub 2017 Feb 21.
INTRODUCTION
We aimed to investigate factors defining amyloid β (1-42) (Aβ) adsorption during preanalytical workup of cerebrospinal fluid (CSF).
METHODS
CSF was transferred to new tubes ≤4 times. Variables tested were different polypropylene tube brands, volumes, CSF Aβ concentrations, incubation times, pipettes, vortex intensities, and other CSF proteins, including hyperphosphorylated tau and Interleukin 1 Receptor Accessory Protein (IL-1RAcP). An enquiry assessed the number of transfers in current practice.
RESULTS
In diagnostic practice, the number of transfers varied between 1 and 3. Every tube transfer resulted in 5% loss of Aβ concentration, even 10% in small volumes. Adsorption was observed after 30 seconds and after contact with the pipette tip. Tube brand, vortexing, or continuous tube movement did not influence adsorption. Adsorption for Aβ was similar, resulting in stable Aβ/Aβ ratios over multiple tube transfers.
DISCUSSION
We confirmed that adsorption of CSF Aβ during preanalytical processing is an important confounder. However, use of the Aβ/Aβ ratio overcomes this effect and can therefore contribute to increased diagnostic accuracy.
简介
我们旨在研究在脑脊液（CSF）的分析前处理过程中定义淀粉样蛋白 β（1-42）（Aβ）吸附的因素。
方法
CSF 被转移到新管中不超过 4 次。测试的变量包括不同的聚丙烯管品牌、体积、CSF Aβ 浓度、孵育时间、移液器、涡旋强度以及其他 CSF 蛋白，包括过度磷酸化的 tau 和白细胞介素 1 受体辅助蛋白（IL-1RAcP）。一项调查评估了当前实践中的转移次数。
结果
在诊断实践中，转移次数在 1 到 3 次之间变化。即使在小体积中，每次管转移都会导致 Aβ 浓度损失 5%，甚至 10%。在 30 秒后观察到吸附，并且在与移液器尖端接触后发生吸附。管品牌、涡旋或连续管运动不会影响吸附。Aβ 的吸附相似，导致在多次管转移过程中 Aβ/Aβ 比值稳定。
讨论
我们证实，在分析前处理过程中 CSF Aβ 的吸附是一个重要的混杂因素。然而，使用 Aβ/Aβ 比值可以克服这种影响，因此可以提高诊断准确性。
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