Hernon Krista M, Whitcomb Tiffany L, Davis Lori, Cooper Timothy K
Department of Comparative Medicine, Penn State University College of Medicine, Milton S Hershey Medical Center, Hershey, Pennsylvania, Preclinical Health, Allegheny Health Network Research Institute, Allegheny Health Network, Pittsburgh, Pennsylvania.
Department of Comparative Medicine, Penn State University College of Medicine, Milton S Hershey Medical Center, Hershey, Pennsylvania.
Comp Med. 2017 Feb 1;67(1):28-33.
A clinically unremarkable 4.5-mo-old female Crl:CD1(ICR) VAF/Elite mouse was euthanized for scheduled sentinel processing. Gross necropsy findings included significant hepatosplenomegaly and visceral lymphadenomegaly, resulting in a preliminary gross diagnosis of lymphoma. Histology revealed florid accumulations of large, 'foamy' macrophages present in the bone marrow, small intestines, and viscera including liver, spleen, lymph nodes, thymus, uterus, and ovaries. The cytoplasm of these cells was abundant, stained pale blue with Wright-Giemsa and was periodic acid-Schiff positive. Given these characteristic gross and histologic findings, a spontaneous lysosomal storage-like disease was diagnosed in this mouse. Cholesterol ester storage disease is likely, because of the visceral involvement with sparing of the CNS, but could not be diagnosed definitively. To our knowledge, this report is the first to describe a case of spontaneous lysosomal storage disease in an outbred mouse of the CD1(ICR) background.
一只4.5月龄、临床无异常的雌性Crl:CD1(ICR) VAF/Elite小鼠因定期哨兵处理而实施安乐死。大体尸检结果包括显著的肝脾肿大和内脏淋巴结肿大，初步大体诊断为淋巴瘤。组织学检查显示，在骨髓、小肠以及包括肝脏、脾脏、淋巴结、胸腺、子宫和卵巢在内的内脏中存在大量“泡沫状”巨噬细胞的丰富聚集。这些细胞的细胞质丰富，经瑞氏-吉姆萨染色呈淡蓝色，且过碘酸-希夫染色呈阳性。鉴于这些特征性的大体和组织学表现，该小鼠被诊断为一种自发性溶酶体贮积样疾病。由于内脏受累而中枢神经系统未受累，很可能是胆固醇酯贮积病，但无法明确诊断。据我们所知，本报告首次描述了一例CD1(ICR)背景的远交小鼠自发性溶酶体贮积病病例。
