Módolo Diego Grando, Araldi Rodrigo Pinheiro, Mazzuchelli-de-Souza Jacqueline, Pereira Alexandre, Pimenta Daniel Carvalho, Zanphorlin Letícia Maria, Beçak Willy, Menossi Marcelo, de Cassia Stocco Rita, de Carvalho Rodrigo Franco
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brazil.
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brazil; Programa de Pós-graduação Interunidades em Biotecnologia, Instituto de Ciências Biomédicas (ICB), Universidade de São Paulo (USP), São Paulo, SP, Brazil.
Vaccine. 2017 Mar 14;35(12):1590-1593. doi: 10.1016/j.vaccine.2017.02.007. Epub 2017 Feb 17.
Bovine papillomatosis is an infectious disease that is caused by bovine papillomavirus (BPV), which results in important economic losses. However, no BPV vaccines or effective treatment methods are commercially available to date. Moreover, the absence of papillomavirus replication in vitro makes the use of recombinant protein a promising candidate for vaccine formulations. Hence, we developed an integrated study on the L1 capsid protein of BPV-1, obtained from a bacterial expression system, regarding its purification, biosafety, thermostability and immunogenicity. The results indicated an absence of genotoxicity of the purified recombinant L1 protein, β-sheet prevalence of secondary structure folding, protein stability under high temperatures as well as the presence of capsomeres and VLPs. In addition, preliminary experimental vaccination of calves showed the production of specific antibodies against BPV-1 L1.
牛乳头瘤病是一种由牛乳头瘤病毒（BPV）引起的传染病，会导致重大经济损失。然而，迄今为止尚无BPV疫苗或有效的治疗方法可供商业使用。此外，乳头瘤病毒在体外无法复制，这使得重组蛋白成为疫苗制剂的一个有前景的候选物。因此，我们开展了一项关于从细菌表达系统获得的BPV-1衣壳蛋白L1的综合研究，涉及该蛋白的纯化、生物安全性、热稳定性和免疫原性。结果表明，纯化的重组L1蛋白没有遗传毒性，二级结构折叠以β-折叠为主，在高温下蛋白稳定，并且存在衣壳粒和病毒样颗粒。此外，对小牛的初步实验性疫苗接种显示产生了针对BPV-1 L1的特异性抗体。
