Al-Salihi Karima A, Al-Dabhawi Ahmed H, Ajeel Ali Abbass, Erzuki Ibrahim A, Ali Tho Alfiqar H
Head of Department of Veterinary Internal Medicine, College of Veterinary Medicine, Al-Muthanna University, Samawah, Iraq.
The Dean of Faculty of Veterinary Medicine, Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Kufa, Najaf, Iraq.
Vet Med Int. 2020 Feb 21;2020:5691974. doi: 10.1155/2020/5691974. eCollection 2020.
The papilloma viruses are constituted of double-stranded DNA and are a more common lesion in ruminant's skin in Iraq. The p53 tumor suppressor protein reveals an essential role in cell cycle control. This study intends to investigate the clinical, histopathological, and immunohistochemical features of cutaneous papilloma in ruminants in Iraq. Samples had been collected from a total of 10 animals (three cattle, three goats, and four sheep) with multiple papillomatosis lesions. The samples were processed for histopathological and immunohistochemical techniques. Clinically, the lesions appeared as multiple various sizes (0.5-11 cm), cauliflower exophytic masses on different parts of the animal's body. The histopathological features of the epidermis granular layer revealed perinuclear vacuolation (koilocytosis) accompanied by various degrees of hypergranulosis, hyperkeratosis, acanthosis, orthokeratosis, and parakeratosis. Strong positive reaction for papillomavirus antigen was seen in both epidermal basal and granular layers in the immunohistochemical investigation (IHC). Moreover, all papilloma lesions revealed an intense positive p53 reaction in cytoplasmic and perinuclear of the basal and parabasal layers. In conclusion, this study described the papillomavirus lesions in bovine, ovine, and caprine animals, which were found in different parts areas of the affected animals. All lesions show similar histopathological features with minor variations. PV antigen and p53 protein expression showed positive results in immunohistochemistry that can be used as diagnostic markers for ruminant's papilloma.
乳头瘤病毒由双链DNA构成，是伊拉克反刍动物皮肤中较为常见的病变。p53肿瘤抑制蛋白在细胞周期控制中发挥着重要作用。本研究旨在调查伊拉克反刍动物皮肤乳头瘤的临床、组织病理学和免疫组织化学特征。从总共10只患有多发性乳头瘤病病变的动物（3头牛、3只山羊和4只绵羊）身上采集样本。对样本进行组织病理学和免疫组织化学技术处理。临床上，病变表现为大小各异（0.5 - 11厘米）的多个菜花状外生性肿块，位于动物身体的不同部位。表皮颗粒层的组织病理学特征显示核周空泡化（挖空细胞形成），伴有不同程度的颗粒层增厚、角化过度、棘层肥厚、正角化和不全角化。在免疫组织化学研究（IHC）中，乳头瘤病毒抗原在表皮基底层和颗粒层均呈现强阳性反应。此外，所有乳头瘤病变在基底层和副基底层的细胞质和核周均显示强烈的p53阳性反应。总之，本研究描述了牛、羊和山羊动物中的乳头瘤病毒病变，这些病变见于受影响动物的不同部位。所有病变均显示出相似的组织病理学特征，略有差异。PV抗原和p53蛋白表达在免疫组织化学中呈阳性结果，可作为反刍动物乳头瘤的诊断标志物。
