Hassanien Rabab T, Hamdy Mervat E, Elnomrosy Sara M, Hussein Heba A, Afify Ahmed F, Darwish Fatma M, Shehab Gehan, Emran Rawhya, Abd-El-Moniem Mervat I I, Habashi Ahmed R, Fahmy Hanan A, Ibraheem Essam M, Shahein Momtaz A, Attya Mohamed, Abdelhakim Ali M M, Hagag Naglaa M
Department of Virology Research, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt.
Genome Research Unit, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt.
Vet World. 2021 Sep;14(9):2296-2305. doi: 10.14202/vetworld.2021.2296-2305. Epub 2021 Sep 3.
Bovine papillomaviruses (BPV) are a heterogeneous group of oncoviruses, distributed globally, which produce major economic losses. In the current study, we compared the results of different diagnostic approaches and compared the strains identified in this study with previously characterized strains at local and international levels.
Samples of skin warts were collected from five bovines with generalized papillomatosis from two Egyptian provinces, Menya and Ismailia, in 2020. Electron microscopy, molecular characterization, histopathological, and immunohistochemical examination were performed.
BPV was detected using electron microscopy in the collected samples. Using molecular characterization, BPV-2 was successfully identified for 1 time in Egypt. The strain has 99.6% identity with the BPV-2 reference strains obtained from GenBank. These results were supported by histopathology and immunohistochemistry examination. Partial nucleotide sequences of the L1 gene were submitted to GenBank with accession numbers MW289843 and MW289844.
BPV-2 was reported for 1 time in the current study. The strain was identified grossly, microscopically, and pathologically and confirmed using molecular approaches. All results were consistent. The sequence analysis revealed that this strain has high sequence similarity to the reference Deltapapillomavirus-4, BPV-2 strains from Brazil and China.
牛乳头瘤病毒（BPV）是一组异质性肿瘤病毒，全球分布，造成重大经济损失。在本研究中，我们比较了不同诊断方法的结果，并将本研究中鉴定的毒株与本地和国际上先前鉴定的毒株进行了比较。
2020年从埃及明亚省和伊斯梅利亚省的5头患有全身性乳头瘤病的牛身上采集皮肤疣样本。进行了电子显微镜检查、分子特征分析、组织病理学和免疫组织化学检查。
通过电子显微镜在采集的样本中检测到BPV。利用分子特征分析，在埃及成功鉴定出1次BPV-2。该毒株与从GenBank获得的BPV-2参考毒株有99.6%的同源性。这些结果得到了组织病理学和免疫组织化学检查的支持。L1基因的部分核苷酸序列已提交至GenBank，登录号为MW289843和MW289844。
本研究中首次报道了BPV-2。该毒株通过大体、显微镜和病理方法鉴定，并使用分子方法进行了确认。所有结果一致。序列分析表明，该毒株与参考Delta乳头瘤病毒-4、来自巴西和中国的BPV-2毒株具有高度序列相似性。
