Diop Khoudia, Diop Awa, Bretelle Florence, Cadoret Frédéric, Michelle Caroline, Richez Magali, Cocallemen Jean-François, Raoult Didier, Fournier Pierre-Edouard, Fenollar Florence
Aix Marseille Univ, Institut Hospitalo-Universitaire Méditerranée-Infection, URMITE, UM63, CNRS 7278, IRD 198, Inserm U1095, Faculté de médecine, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France.
Aix Marseille Univ, Institut Hospitalo-Universitaire Méditerranée-Infection, URMITE, UM63, CNRS 7278, IRD 198, Inserm U1095, Faculté de médecine, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France; Department of Gynecology and Obstetrics, Gynépole, Marseille, Pr Boubli et D'Ercole, Hôpital Nord, Assistance Publique-Hôpitaux de Marseille, AMU, Aix-Marseille Université, France.
Anaerobe. 2017 Apr;44:87-95. doi: 10.1016/j.anaerobe.2017.02.012. Epub 2017 Feb 20.
Strain KHD7, a Gram-stain-positive rod-shaped, non-sporulating, strictly anaerobic bacterium, was isolated from the vaginal swab of a woman with bacterial vaginosis. We studied its phenotypic characteristics and sequenced its complete genome. The major fatty acids were C16:0 (44%), C18:2n6 (22%), and C18:1n9 (14%). The 1,806,744 bp long genome exhibited 49.24% G+C content; 1549 protein-coding and 51 RNA genes. Strain KHD7 exhibited a 93.5% 16S rRNA similarity with Olsenella uli, the phylogenetically closest species in the family Coriobacteriaceae. Therefore, strain KHD7 is sufficiently distinct to represent a new genus, for which we propose the name Olegusella massiliensis gen. nov., sp. nov. The type strain is KHD7.
菌株KHD7是一种革兰氏染色阳性的杆状、无芽孢、严格厌氧的细菌，从一名患有细菌性阴道病的女性阴道拭子中分离得到。我们研究了其表型特征并对其全基因组进行了测序。主要脂肪酸为C16:0（44%）、C18:2n6（22%）和C18:1n9（14%）。该基因组长度为1,806,744 bp，G+C含量为49.24%；有1549个蛋白质编码基因和51个RNA基因。菌株KHD7与科里杆菌科中系统发育关系最密切的物种乌利奥尔森氏菌（Olsenella uli）的16S rRNA相似度为93.5%。因此，菌株KHD7具有足够的独特性，代表一个新属，我们为此提议将其命名为马西利亚奥列古氏菌（Olegusella massiliensis），新属，新种。模式菌株为KHD7。
