Elsawi Ziena, Togo Amadou Hamidou, Beye Mamadou, Dubourg Grégory, Andrieu Claudia, Armsrtong Nicholas, Richez Magali, di Pinto Fabrizio, Bittar Fadi, Labas Noémie, Fournier Pierre-Edouard, Raoult Didier, Khelaifia Saber
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, CNRS (UMR 7278), IRD (198), INSERM (U1095), AMU (UM63), Institut Hospitalo-Universitaire Méditerranée-Infection, Faculté de médecine, Aix-Marseille Université, Marseille, France.
Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
Microbiologyopen. 2017 Aug;6(4). doi: 10.1002/mbo3.458. Epub 2017 Mar 21.
The human gut is composed of a large diversity of microorganisms, which have been poorly described. Here, using culturomics, a new concept based on the variation in culture conditions and MALDI-TOF MS identification, we proceed to explore the microbial diversity of the complex ecosystem of the human gut. Using this approach, we isolated strain AT8 (=CSUR P2118 = DSM 101782) from stool specimens collected from a 51-year-old obese French woman. Strain AT8 is a strictly anaerobic, nonmotile, nonspore-forming gram-positive coccus that do not exhibit catalase and oxidase activities. 16S rDNA-based identification of strain AT8 demonstrated 92% gene sequence similarity with Eggerthella lenta DSM 2243, the phylogenetically closed validly named type species. Here, we present a set of features for the strain AT8 and the description of its complete genome sequence and annotation. The 2,091,845 bp long genome has a G+C content of 63.46% and encodes1,849 predicted genes; 1,781 were protein-coding genes, and 68 were RNAs. On the basis of the characteristics reported here, we propose the creation of a new bacterial genus Hugonella gen. nov., belonging to the Eggerthellaceae family and including Hugonella massiliensis gen. nov., sp. nov., strain AT8 as the type strain.
人类肠道由种类繁多的微生物组成，对其描述甚少。在此，我们运用培养组学这一基于培养条件变化和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定的新概念，着手探索人类肠道复杂生态系统中的微生物多样性。采用这种方法，我们从一名51岁肥胖法国女性的粪便标本中分离出菌株AT8（=CSUR P2118 = DSM 101782）。菌株AT8是一种严格厌氧、无运动性、不形成芽孢的革兰氏阳性球菌，不表现出过氧化氢酶和氧化酶活性。基于16S rDNA对菌株AT8的鉴定表明，其与迟缓埃格特菌DSM 2243的基因序列相似性为92%，后者是系统发育关系密切的有效命名模式种。在此，我们展示了菌株AT8的一系列特征，并对其完整基因组序列及注释进行了描述。该基因组长度为2,091,845 bp，G+C含量为63.46%，编码1,849个预测基因；其中1,781个为蛋白质编码基因，68个为RNA。基于本文报道的特征，我们提议创建一个新的细菌属——胡戈菌属（Hugonella gen. nov.），属于埃格特菌科（Eggerthellaceae），包括马赛胡戈菌（Hugonella massiliensis gen. nov., sp. nov.），菌株AT8为模式菌株。
