Zhang Xinqi, Yang Mingxi, Shi Hua, Hu Jianxin, Wang Yuanlin, Sun Zhaolin, Xu Shuxiong
Emergency Department, General Hospital of Jinan Military Area, Jinan, Shandong, 250031, China.
Department of Urology, Guizhou Provincial People's Hospital Affiliated to Guizhou Medical University, Guiyang, Guizhou, 550002, China.
Oncotarget. 2017 Mar 21;8(12):19566-19576. doi: 10.18632/oncotarget.15361.
Reduced expression of E-cadherin was observed in renal cell carcinoma (RCC). However, its potential clinical value and correlation with WNT/β-catenin signaling in RCC progression was still unclear. Immunohistochemical staining was performed in RCC tissue microarray to examine the expression status and prognosis value of E-cadherin and β-catenin. The potential role of E-cadherin in β-catenin translocation was analyzed with immunobloting assays. A significant negative correlation was observed between E-cadherin and β-catenin expression in RCC tissues. E-cadherin inhibits β-catenin translocation from membrane to cytoplasm in RCC tissues, which was an important step for WNT/β-catenin signaling. Reduced E-cadherin expression was associated with poor prognosis. More importantly, E-cadherin-/β-catenin+ was an independent detrimental factor for survival estimation of RCC patients. Reduced E-cadherin expression in RCC promoted cancer progression via WNT/β-catenin signaling pathway activation. E-cadherin/β-catenin provides a valuable prognosis marker for RCC, which may be an effective target for RCC therapy.
在肾细胞癌（RCC）中观察到E-钙黏蛋白表达降低。然而，其潜在的临床价值以及与RCC进展中WNT/β-连环蛋白信号传导的相关性仍不清楚。在RCC组织芯片上进行免疫组织化学染色，以检测E-钙黏蛋白和β-连环蛋白的表达状态及预后价值。用免疫印迹分析检测E-钙黏蛋白在β-连环蛋白易位中的潜在作用。在RCC组织中观察到E-钙黏蛋白与β-连环蛋白表达之间存在显著负相关。E-钙黏蛋白抑制RCC组织中β-连环蛋白从细胞膜向细胞质的易位，这是WNT/β-连环蛋白信号传导的重要步骤。E-钙黏蛋白表达降低与预后不良相关。更重要的是，E-钙黏蛋白阴性/β-连环蛋白阳性是RCC患者生存评估的独立不利因素。RCC中E-钙黏蛋白表达降低通过激活WNT/β-连环蛋白信号通路促进癌症进展。E-钙黏蛋白/β-连环蛋白为RCC提供了一个有价值的预后标志物，可能是RCC治疗的有效靶点。
