Department of Pathology, Faculty of Medicine, Helwan University, Helwan 11795, Egypt.
Department of anatomical Pathology, Faculty of Medicine, Cairo University, Cairo 12613, Egypt.
Asian Pac J Cancer Prev. 2024 Nov 1;25(11):4097-4107. doi: 10.31557/APJCP.2024.25.11.4097.
Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is known to be upregulated in the tumors with ability to metastasize. In contrast, long non-coding Ribonuclei acid (lncRNA) MALAT1 was reported by some studies to be downregulated in meningioma cells. E-cadherin and Cyclin D1 are prognostic indicators and possible attractive targets for the treatment of recurring and aggressive meningioma. This study aimed to evaluate the expression level of lncRNA MALAT1, as well as Cyclin D1 and E-cadherin immunohistochemical expression in meningiomas (Grade 1 and 2) and their association with the clinicopathological parameters of the studied cases.
Quantitative determination of relative expression levels of lncRNA-MALAT1 in 64 cases of meningioma to 5 controls of normal dura mater was performed, in addition to evaluation of E-cadherin and Cyclin D1 immunohistochemical expression. The results were tested for association with the clinicopathological parameters.
lncRNA-MALAT1 expression were downregulated in 49/64 of the cases of meningioma (76%) in comparison to control. There were significant association of expression of lncRNA-MALAT1 with grade, brain invasion and increased mitosis (p=0.007, 0.04, 0.006 respectively). There were also significant associations of strong E-cadherin and negative Cyclin D1 proteins expression with grade 1 (p = 0.02, 0.004), low mitosis (p=0.03, and 0.04) and brain invasion (p=0.04, 0.03) respectively. Additionally, a significant weak negative correlation between E-cadherin and Cyclin D1 expression was fond, yet no significant correlation between lncRNA MALAT1 expression and either of E-cadherin or Cyclin D1 expression could be achieved.
lncRNA MALAT1 is downregulated in meningiomas and associated with increased aggressiveness. Overexpressed cyclin D1 and decreased E-cadherin expression are also associated with high grade meningioma.
转移相关肺腺癌转录本 1（MALAT1）已知在具有转移能力的肿瘤中上调。相比之下，一些研究报道长非编码 RNA（lncRNA）MALAT1在脑膜瘤细胞中下调。E-钙黏蛋白和细胞周期蛋白 D1 是脑膜瘤（1 级和 2 级）的预后指标和可能有吸引力的治疗靶点，用于治疗复发性和侵袭性脑膜瘤。本研究旨在评估 lncRNA MALAT1 的表达水平，以及 E-钙黏蛋白和细胞周期蛋白 D1 的免疫组织化学表达在脑膜瘤中的表达，并将其与研究病例的临床病理参数相关联。
对 64 例脑膜瘤和 5 例正常硬脑膜对照的 lncRNA-MALAT1 相对表达水平进行定量测定，同时评估 E-钙黏蛋白和细胞周期蛋白 D1 的免疫组织化学表达。对结果进行了与临床病理参数的相关性检验。
与对照组相比，64 例脑膜瘤中有 49 例（76%）的 lncRNA-MALAT1 表达下调。lncRNA-MALAT1 的表达与分级、脑侵袭和有丝分裂增加有显著相关性（p=0.007、0.04、0.006）。E-钙黏蛋白强表达和细胞周期蛋白 D1 蛋白表达阴性与 1 级（p=0.02、0.004）、低有丝分裂（p=0.03、0.04）和脑侵袭（p=0.04、0.03）也有显著相关性。此外，还发现 E-钙黏蛋白和细胞周期蛋白 D1 表达之间存在显著的负相关，但 lncRNA MALAT1 表达与 E-钙黏蛋白或细胞周期蛋白 D1 表达之间没有显著相关性。
lncRNA MALAT1 在脑膜瘤中下调，与侵袭性增加相关。过表达的细胞周期蛋白 D1 和下调的 E-钙黏蛋白表达也与高级别脑膜瘤相关。
