Schneider A, Meinhardt G, De-Villiers E M, Gissmann L
Department of Obstetrics and Gynecology, University of Ulm, West Germany.
Diagn Cytopathol. 1987 Sep;3(3):250-5. doi: 10.1002/dc.2840030315.
The cytologic diagnosis of cervical condyloma is based on criteria developed over the last 10 years. It has now become possible to document the presence of human papilloma virus (HPV) DNA directly in cervical swabs by the highly sensitive technique of DNA filter hybridization in situ. The purpose of this article is to evaluate critically the empirically established cytologic criteria of condyloma by comparing them with HPV-DNA hybridization studies in the same material. The results of this study indicate that "classic" koilocytosis and dyskeratocytosis are not highly sensitive criteria for the presence of HPV infection, identifying only 15% of the HPV-DNA-positive cases correctly. In an attempt to improve the sensitivity of the cytologic diagnosis of HPV infections, a panel of nine "nonclassic" criteria was evaluated. The five most valuable signs were "mild koilocytosis," mild dyskeratocytosis," hyperchromatic nuclei, bi- and multinucleation, and cleared cytoplasm. Using these criteria in combination, statistically discriminant analysis could correctly identify 84% of the HPV-positive group.
宫颈尖锐湿疣的细胞学诊断基于过去10年制定的标准。通过DNA滤膜原位杂交这种高度敏感的技术，现在已能够直接在宫颈拭子中检测人乳头瘤病毒（HPV）DNA的存在。本文的目的是通过将经验性建立的尖锐湿疣细胞学标准与同一材料中的HPV - DNA杂交研究进行比较，对其进行严格评估。这项研究的结果表明，“经典”的挖空细胞和角化不良细胞并非HPV感染的高度敏感标准，仅能正确识别15%的HPV - DNA阳性病例。为提高HPV感染细胞学诊断的敏感性，对一组九个“非经典”标准进行了评估。五个最有价值的体征是“轻度挖空细胞”、“轻度角化不良细胞”、核深染、双核和多核以及胞质清亮。综合使用这些标准，统计判别分析能够正确识别84%的HPV阳性组。
