Tahir Hafiz A S, Gu Qin, Wu Huijun, Raza Waseem, Hanif Alwina, Wu Liming, Colman Massawe V, Gao Xuewen
Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Diseases and Pests, Ministry of Education Nanjing, China.
Jiangsu Key Lab for Organic Solid Waste Utilization, Nanjing Agricultural University Nanjing, China.
Front Microbiol. 2017 Feb 7;8:171. doi: 10.3389/fmicb.2017.00171. eCollection 2017.
Bacterial volatiles play a significant role in promoting plant growth by regulating the synthesis or metabolism of phytohormones. and growth chamber experiments were conducted to investigate the effect of volatile organic compounds (VOCs) produced by the plant growth promoting rhizobacterium strain SYST2 on hormone regulation and growth promotion in tomato plants. We observed a significant increase in plant biomass under both experimental conditions; we observed an increase in photosynthesis and in the endogenous contents of gibberellin, auxin, and cytokinin, while a decrease in ethylene levels was noted. VOCs emitted by SYST2 were identified through gas chromatography-mass spectrometry analysis. Of 11 VOCs tested in glass jars containing plants in test tubes, only two, albuterol and 1,3-propanediole, were found to promote plant growth. Furthermore, tomato plants showed differential expression of genes involved in auxin (), gibberellin (), cytokinin (), expansin (), and ethylene () biosynthesis or metabolism in roots and leaves in response to SYST2 VOCs. Our findings suggest that SYST2-derived VOCs promote plant growth by triggering growth hormone activity, and provide new insights into the mechanism of plant growth promotion by bacterial VOCs.
细菌挥发物通过调节植物激素的合成或代谢在促进植物生长方面发挥着重要作用。进行了生长室实验，以研究植物促生根际细菌SYST2产生的挥发性有机化合物（VOCs）对番茄植株激素调节和生长促进的影响。在两种实验条件下，我们都观察到植物生物量显著增加；我们观察到光合作用以及赤霉素、生长素和细胞分裂素的内源含量增加，同时乙烯水平下降。通过气相色谱-质谱分析鉴定了SYST2排放的VOCs。在装有试管中植物的玻璃瓶中测试的11种VOCs中，仅发现沙丁胺醇和1,3-丙二醇这两种物质能促进植物生长。此外，番茄植株的根和叶中参与生长素、赤霉素、细胞分裂素、扩展蛋白和乙烯生物合成或代谢的基因在响应SYST2 VOCs时表现出差异表达。我们的研究结果表明，SYST2衍生的VOCs通过触发生长激素活性促进植物生长，并为细菌VOCs促进植物生长的机制提供了新的见解。
