青枯雷尔氏菌产生的挥发性化合物对芽孢杆菌挥发性物质促进植物生长和诱导系统抗性潜力的影响。
Effect of volatile compounds produced by Ralstonia solanacearum on plant growth promoting and systemic resistance inducing potential of Bacillus volatiles.
作者信息
Tahir Hafiz Abdul Samad, Gu Qin, Wu Huijun, Raza Waseem, Safdar Asma, Huang Ziyang, Rajer Faheem Uddin, Gao Xuewen
机构信息
Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Weigang No.1, Nanjing, 210095, People's Republic of China.
Plant Pathology section, Tobacco Research station, Pakistan Tobacco Board, Ministry of Commerce, Govt. of Pakistan, Hayatabad, Peshawar, Pakistan.
出版信息
BMC Plant Biol. 2017 Aug 2;17(1):133. doi: 10.1186/s12870-017-1083-6.
BACKGROUND
Microbial volatiles play an expedient role in the agricultural ecological system by enhancing plant growth and inducing systemic resistance against plant pathogens, without causing hazardous effects on the environment. To explore the effects of VOCs of Ralstonia solanacearum TBBS1 (Rs) on tobacco plant growth and on plant growth promoting efficiency of VOCs produced by Bacillus subtilis SYST2, experiments were conducted both in vitro and in planta.
RESULTS
The VOCs produced by SYST2 significantly enhanced the plant growth and induced the systemic resistance (ISR) against wilt pathogen Rs in all experiments. The SYST2-VOCs significantly increased PPO and PAL activity and over-expressed the genes relating to expansin, wilt resistance, and plant defense while repressed the genes relating to ethylene production. More interestingly, VOCs produced by pathogen, Rs had no significant effect on plant growth; however, Rs-VOCs decreased the growth promoting potential of SYST2-VOCs when plants were exposed to VOCs produced by both SYST2 and Rs. The co-culture of SYST2 and Rs revealed that they inhibited the growth of each other; however, the inhibition of Rs by SYST2-VOCs appeared to be greater than that of SYST2 by Rs-VOCs.
CONCLUSION
Our findings provide new insights regarding the interaction among SYST2-VOCs, Rs-VOCs and plant, resulting in growth promotion and induced systemic resistance against the bacterial wilt pathogen Rs. This is the first report of the effect of VOCs produced by pathogenic microorganism on plant growth and on plant growth-promoting and systemic resistance-inducing potential of PGPR strain SYST2.
背景
微生物挥发物在农业生态系统中发挥着有利作用，可促进植物生长并诱导对植物病原体的系统抗性，且不会对环境造成有害影响。为了探究青枯雷尔氏菌TBBS1（Rs）的挥发性有机化合物（VOCs）对烟草植株生长以及枯草芽孢杆菌SYST2产生的VOCs促进植物生长效率的影响，我们进行了体外和体内实验。
结果
在所有实验中，SYST2产生的VOCs均显著促进了植物生长，并诱导了对枯萎病原菌Rs的系统抗性（ISR）。SYST2-VOCs显著提高了多酚氧化酶（PPO）和苯丙氨酸解氨酶（PAL）的活性，使与扩张蛋白、抗枯萎病和植物防御相关的基因过度表达，同时抑制了与乙烯产生相关的基因。更有趣的是，病原菌Rs产生的VOCs对植物生长没有显著影响；然而，当植物暴露于SYST2和Rs产生的VOCs时，Rs-VOCs降低了SYST2-VOCs促进生长的潜力。SYST2和Rs的共培养表明它们相互抑制生长；然而，SYST2-VOCs对Rs的抑制作用似乎大于Rs-VOCs对SYST2的抑制作用。
结论
我们的研究结果为SYST2-VOCs、Rs-VOCs与植物之间的相互作用提供了新的见解，这种相互作用导致了植物生长促进以及对细菌性枯萎病原菌Rs的系统抗性诱导。这是关于病原微生物产生的VOCs对植物生长以及PGPR菌株SYST2促进植物生长和诱导系统抗性潜力影响的首次报道。
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