Wang J Z, Xu C L, Wu H, Shen S J
Department of Gastroenterology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Braz J Med Biol Res. 2017 Feb 20;50(3):e6079. doi: 10.1590/1414-431X20176079.
Several long non-coding RNA (lncRNA) might be correlated with the prognosis of colorectal cancer (CRC) and serve as a diagnostic and prognostic biomarker. However, the exact expression pattern of small nucleolar RNA host gene 12 (SNHG12) in colorectal cancer and its clinical significance remains unclear. The level of SNHG12 was detected by qRT-PCR in CRC tissues and CRC cells. MTT assay and colony formation assay were performed to examine the cell proliferation of CRC cells transfected with pcDNA-SNHG12 or si-SNHG12. Flow cytometry technology was used to detect cell cycle and cell apoptosis of CRC cells transfected with pcDNA-SNHG12 or si-SNHG12. The protein level of cell cycle progression-related molecules, including cyclin-dependent kinases (CDK4, CDK6), cyclin D1 (CCND1) and cell apoptosis-related molecule caspase 3 was detected by western blot. The effect of SNHG12 knockdown was examined in vivo. Increased levels of SNHG12 were observed in CRC tissues and in CRC cells. SNHG12 promoted the cell proliferation of CRC cells. In addition, SNHG12 overexpression boosted the cell cycle progression of SW480 cells transfected with pcDNA-SNHG12 and SNHG12 knockdown inhibited the cell cycle progression of HT29 cells transfected with si-SNHG12. SNHG12 also inhibited the cell apoptosis of CRC cells. We also found that SNHG12 increased the expression of cell cycle-related proteins and suppressed the expression of caspase 3. Our results suggest that SNHG12 promoted cell growth and inhibited cell apoptosis in CRC cells, indicating that SNHG12 might be a useful biomarker for colorectal cancer.
几种长链非编码RNA（lncRNA）可能与结直肠癌（CRC）的预后相关，并可作为诊断和预后生物标志物。然而，小核仁RNA宿主基因12（SNHG12）在结直肠癌中的具体表达模式及其临床意义仍不清楚。采用qRT-PCR检测CRC组织和CRC细胞中SNHG12的水平。进行MTT法和集落形成试验，以检测转染pcDNA-SNHG12或si-SNHG12的CRC细胞的增殖情况。采用流式细胞术检测转染pcDNA-SNHG12或si-SNHG12的CRC细胞的细胞周期和细胞凋亡情况。通过蛋白质印迹法检测细胞周期进程相关分子的蛋白水平，包括细胞周期蛋白依赖性激酶（CDK4、CDK6）、细胞周期蛋白D1（CCND1）和细胞凋亡相关分子半胱天冬酶3。在体内检测SNHG12敲低的效果。在CRC组织和CRC细胞中观察到SNHG12水平升高。SNHG12促进CRC细胞的增殖。此外，SNHG12过表达促进了转染pcDNA-SNHG12的SW480细胞的细胞周期进程，而SNHG12敲低抑制了转染si-SNHG12的HT29细胞的细胞周期进程。SNHG12还抑制CRC细胞的凋亡。我们还发现SNHG12增加了细胞周期相关蛋白的表达，并抑制了半胱天冬酶3的表达。我们的结果表明，SNHG12促进CRC细胞的生长并抑制其凋亡，这表明SNHG12可能是结直肠癌的一个有用生物标志物。
