Department of Thoracic Surgery, Fujian Medical University Union Hospital, #29 Xinquan Road, 350001, Fuzhou, Fujian, China.
Department of Central Laboratory, Fujian Medical University Union Hospital, Fuzhou, Fujian, China.
Clin Transl Oncol. 2020 Oct;22(10):1786-1795. doi: 10.1007/s12094-020-02317-7. Epub 2020 Feb 21.
Esophageal squamous cell cancer (ESCC) has high rates of recurrence and mortality. Small nucleolar RNA host gene 12 (SNHG12) is known to promote the progression of several cancers. Therefore, we aimed to investigate the expression and role of SNHG12 in ESCC.
The expression and clinical value of SNHG12 in esophageal cancer were explored using data from The Cancer Genome Atlas (TCGA) and the online server GEPIA. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to verify the expression levels of SNHG12 in ESCC tissues and cell lines. Furthermore, loss-of-function assays were performed to examine the effect of SNHG12 on ESCC cells in vitro and in vivo. The potential competing endogenous RNA networks of SNHG12 in ESCC were explored.
SNHG12 was downregulated in human ESCA tissues compared to control tissues. The expression of SNHG12 was strongly associated with T stage, N stage, and TNM stage. Low SNHG12 expression in esophageal tumor tissues was significantly correlated with poor prognosis. Furthermore, knockdown of SNHG12 not only promoted proliferation, colony formation, migration, and invasion and inhibited apoptosis in ESCC cells in vitro, but also increased tumor growth in vivo. Additionally, this proves that the SNHG12/miRNA-195-5p/BCL9 network might be involved in ESCC.
This is the first study to reveal that SNHG12 is downregulated in ESCC tissues and could be used as a prognostic tool. SNHG12 suppressed tumor progression in ESCC cells, serving as a potential biomarker. The SNHG12/miRNA-195-5p/BCL9 network is proposed to be the mechanism leading to ESCC progression.
食管鳞状细胞癌（ESCC）具有较高的复发和死亡率。小核仁 RNA 宿主基因 12（SNHG12）已知可促进几种癌症的进展。因此，我们旨在研究 SNHG12 在 ESCC 中的表达和作用。
利用癌症基因组图谱（TCGA）和在线服务器 GEPIA 中的数据，探讨了 SNHG12 在食管癌中的表达和临床价值。实时定量聚合酶链反应（qRT-PCR）用于验证 SNHG12 在 ESCC 组织和细胞系中的表达水平。此外，进行了功能丧失实验，以研究 SNHG12 对 ESCC 细胞在体外和体内的影响。探索了 SNHG12 在 ESCC 中的潜在竞争内源 RNA 网络。
与对照组织相比，SNHG12 在人 ESCA 组织中下调。SNHG12 的表达与 T 分期、N 分期和 TNM 分期密切相关。食管肿瘤组织中 SNHG12 低表达与预后不良显著相关。此外，SNHG12 的敲低不仅促进了 ESCC 细胞在体外的增殖、集落形成、迁移和侵袭，还抑制了细胞凋亡，并且在体内增加了肿瘤生长。此外，这证明了 SNHG12/miRNA-195-5p/BCL9 网络可能参与了 ESCC。
这是第一项表明 SNHG12 在 ESCC 组织中下调并可作为预后工具的研究。SNHG12 抑制 ESCC 细胞的肿瘤进展，可作为潜在的生物标志物。提出了 SNHG12/miRNA-195-5p/BCL9 网络是导致 ESCC 进展的机制。
