Giordano Alessia, Rossi Gabriele, Probo Monica, Moretti Pierangelo, Paltrinieri Saverio
Department of Veterinary Medicine, University of Milan, Via Celoria, 10-20133 Milan, Italy; Central Laboratory, Veterinary Teaching Hospital - University of Milan, Via dell'Università, 6, 26900 Lodi, Italy.
Department of Veterinary Medicine, University of Milan, Via Celoria, 10-20133 Milan, Italy; College of Veterinary Medicine, School of Veterinary and Life Science, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia.
Res Vet Sci. 2017 Apr;111:108-112. doi: 10.1016/j.rvsc.2017.02.007. Epub 2017 Feb 13.
High density lipoproteins (HDLs) are pivotal in innate immunity and decrease in serum during inflammation. Several studies have been done about lipoprotein changes in transition cows but little is known about their changes in newborn calves. The aim of this study is to provide information about HDLs in newborn calves, by defining the possible age-related changes in healthy calves compared with adults and by assessing the possible differences in calves with inflammation. Lipoprotein electrophoretic separation (reported as percentages) and colorimetric measurement of HDL (HDL-C) were performed on healthy cows and calves in order to identify possible differences in the lipoprotein profile due to the age. Then, age-matched calves with inflammatory conditions were also evaluated. Results showed that in calves HDL% and VLDL% were lower (mean values±SD: 77.6%±8.6% and 2.6%±2.5%, respectively) and LDL% was higher (19.7%±7.4%) than in adults (89.0%±3.9%; 5.2±2.1% and 5.8%±3.1%, respectively). Sick calves revealed a decrease of both HDL% (mean values ± SD: 61.0%±22.1%) and HDL-C (22.8±11.6mg/dL) and an increase of VLDL% (12.1% ±13.1%) compared with controls (77.6%±8.6%; 41.5±11.2mg/dL and 2.6%±2.5%, respectively). Paraoxonase-1 activity, influenced by inflammation and oxidation, was measured, and it appeared correlated with HDL% and HDL-C in sick calves. In conclusion, this study revealed that HDLs concentration in healthy calves is lower than in adults, and further decreases in calves with inflammation, likely due to oxidation.
高密度脂蛋白（HDL）在先天免疫中起关键作用，且在炎症期间血清中含量会降低。关于围产母牛脂蛋白变化已开展了多项研究，但对于新生犊牛脂蛋白变化的了解却很少。本研究的目的是通过确定健康犊牛与成年牛相比可能存在的与年龄相关的变化，以及评估炎症状态下犊牛的可能差异，来提供有关新生犊牛HDL的信息。对健康母牛和犊牛进行脂蛋白电泳分离（以百分比表示）和HDL（HDL-C）比色测定，以确定由于年龄导致的脂蛋白谱可能存在的差异。然后，对年龄匹配的患有炎症的犊牛也进行了评估。结果显示，犊牛的HDL%和极低密度脂蛋白（VLDL）%低于成年牛（平均值±标准差：分别为77.6%±8.6%和2.6%±2.5%），而低密度脂蛋白（LDL）%高于成年牛（分别为19.7%±7.4%、89.0%±3.9%；5.2±2.1%和5.8%±3.1%）。患病犊牛的HDL%（平均值±标准差：61.0%±22.1%）和HDL-C（22.8±11.6mg/dL）均降低，VLDL%（12.1% ±13.1%）升高，而对照组分别为77.6%±8.6%；41.5±11.2mg/dL和2.6%±2.5%。测定了受炎症和氧化影响的对氧磷酶-1活性，结果显示其与患病犊牛的HDL%和HDL-C相关。总之，本研究表明健康犊牛的HDL浓度低于成年牛，且炎症状态下的犊牛HDL浓度会进一步降低，这可能是由于氧化作用所致。
