基因从大肠杆菌转移至弯曲杆菌属：用于空肠弯曲杆菌遗传分析的穿梭载体的开发
Gene transfer from Escherichia coli to Campylobacter species: development of shuttle vectors for genetic analysis of Campylobacter jejuni.
作者信息
Labigne-Roussel A, Harel J, Tompkins L
机构信息
Division of Infectious Diseases, Stanford University, California 95305.
出版信息
J Bacteriol. 1987 Nov;169(11):5320-3. doi: 10.1128/jb.169.11.5320-5323.1987.
Abstract
A shuttle cloning vector (pIL550) has been constructed which can be mobilized from Escherichia coli to Campylobacter jejuni, Campylobacter coli, and Campylobacter fetus by complementation with the transfer functions of an IncP plasmid in trans, with a frequency of 10(-4) transconjugants per donor. We also present evidence for a DNA modification system in C. jejuni.
摘要
构建了一种穿梭克隆载体（pIL550），通过与IncP质粒的转移功能在反式中互补，可从大肠杆菌转移至空肠弯曲菌、结肠弯曲菌和胎儿弯曲菌，转移频率为每供体10^(-4) 个转接合子。我们还提供了空肠弯曲菌中存在DNA修饰系统的证据。
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