Lambert T, Gerbaud G, Trieu-Cuot P, Courvalin P
Ann Inst Pasteur Microbiol (1985). 1985 Sep-Oct;136B(2):135-50. doi: 10.1016/s0769-2609(85)80040-5.
Campylobacter coli strain BM2509 resistant to ampicillin, chloramphenicol, erythromycin, kanamycin, spectinomycin, streptomycin and tetracycline was isolated from a patient with hospital-acquired diarrhoea. Resistance to kanamycin has not been thus far described in Campylobacter. Phosphocellulose paper-binding assay indicated that resistance to kanamycin and structurally related antibiotics in strain BM2509 was due to synthesis of a 3'-aminoglycoside phosphotransferase of type III (APH(3')-III), an enzyme so far confined to Gram-positive cocci. The kanamycin and tetracycline resistances were transferable en bloc by conjugation to C. fetus but not to Escherichia coli. Analysis by agarose gel electrophoresis of crude bacterial lysates revealed the presence, in BM2509 and in the transconjugants, of a plasmid, pIP1433, with a size of 47.2 kilobases (Kb). Strain BM2509 also harboured a 4.5-Kb cryptic plasmid. DNA annealing studies indicated a close structural relationship between the kanamycin resistance gene of C. coli BM2509 and that representative of this type of resistance determinant in Gram-positive cocci. These results indicate that emergence of resistance to kanamycin in Campylobacter is due to acquisition in vivo of a gene or a plasmid from Gram-positive bacteria.
从一名医院获得性腹泻患者中分离出了对氨苄西林、氯霉素、红霉素、卡那霉素、壮观霉素、链霉素和四环素耐药的空肠弯曲菌菌株BM2509。空肠弯曲菌对卡那霉素的耐药性此前尚未见报道。磷酸纤维素纸结合试验表明，菌株BM2509对卡那霉素及结构相关抗生素的耐药性是由于合成了III型3'-氨基糖苷磷酸转移酶（APH(3')-III），该酶迄今仅存在于革兰氏阳性球菌中。卡那霉素和四环素耐药性可通过接合作用整体转移至胎儿弯曲菌，但不能转移至大肠杆菌。对粗制细菌裂解物进行琼脂糖凝胶电泳分析显示，BM2509及其接合子中存在一个大小为47.2千碱基（Kb）的质粒pIP1433。菌株BM2509还含有一个4.5 Kb的隐蔽质粒。DNA退火研究表明，大肠杆菌BM2509的卡那霉素耐药基因与革兰氏阳性球菌中此类耐药决定簇的代表基因在结构上密切相关。这些结果表明，空肠弯曲菌对卡那霉素耐药性的出现是由于在体内从革兰氏阳性细菌获得了一个基因或质粒。
