Sibille Y, Naegel G P, Merrill W W, Young K R, Care S B, Reynolds H Y
Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510.
J Lab Clin Med. 1987 Nov;110(5):624-33.
Activated macrophages can secrete a number of mediators that can attract inflammatory cells and enhance secretion of phlogistic substances from these cells. The ultimate effect of activated bronchoalveolar lavage (BAL) cells may be fibrotic lung injury. Inasmuch as pulmonary sarcoidosis is a disease associated with spontaneous activation of macrophages and lymphocytes among BAL cells, cells obtained from patients with sarcoidosis were compared with normal cells. We report that adherent BAL cells in culture from patients with sarcoidosis (n = 21) release during a resting period in vitro more chemotactic activity for neutrophils (PMNs) than do BAL cells from normal individuals (n = 14). After density fractionation of the respiratory cells by albumin gradient, cells from high-density fractions in the group with sarcoidosis secrete more chemotactic activity for neutrophils than cells from less dense fractions. The PMN chemotactic activity spontaneously released in vitro by BAL cells from patients with sarcoidosis correlates with the percentage of PMNs recovered by BAL. Immunochemical bioassay and high-performance liquid chromatographic (HPLC) analysis of BAL cell supernatants revealed a complex pattern of chemotactic factors to be present. Generally, three peaks of chemotactic activity were noted on HPLC 1-60 separations at greater than 20 kd, 8 to 10 kd, and less than 1 kd apparent molecular weights. Significantly, interleukin-1 was present in these supernatants, whereas complement components and leukotriene B4 were absent. Sarcoid BAL cells, principally alveolar macrophages, are activated in vivo as manifested by spontaneous secretion of chemotactic factors for PMNs in vitro. Interleukin-1 and other less well characterized molecules were detected. The presence of PMNs among the lavage cells of some patients with sarcoidosis appears to be an in vivo biologic correlate of this activation. These data provide additional criteria of BAL cell activation in patients with pulmonary sarcoidosis and provide further evidence concerning factors that attract inflammatory cells into the lung.
活化的巨噬细胞可分泌多种介质，这些介质能吸引炎症细胞并增强这些细胞炎症物质的分泌。活化的支气管肺泡灌洗（BAL）细胞的最终效应可能是肺纤维化损伤。鉴于肺结节病是一种与BAL细胞中巨噬细胞和淋巴细胞自发活化相关的疾病，因此将结节病患者的细胞与正常细胞进行了比较。我们报告，结节病患者（n = 21）体外培养的贴壁BAL细胞在静息期释放的对中性粒细胞（PMN）的趋化活性比正常个体（n = 14）的BAL细胞更高。通过白蛋白梯度对呼吸细胞进行密度分级后，结节病组高密度级分的细胞比低密度级分的细胞分泌更多对中性粒细胞的趋化活性。结节病患者BAL细胞体外自发释放的PMN趋化活性与BAL回收的PMN百分比相关。对BAL细胞上清液进行免疫化学生物测定和高效液相色谱（HPLC）分析，发现存在复杂的趋化因子模式。一般来说，在HPLC 1 - 60分离中，在大于20 kd、8至10 kd和小于1 kd的表观分子量处观察到三个趋化活性峰。值得注意的是，这些上清液中存在白细胞介素-1，而补体成分和白三烯B4不存在。结节病BAL细胞，主要是肺泡巨噬细胞，在体内被激活，体外表现为自发分泌对PMN的趋化因子。检测到白细胞介素-1和其他特征不太明确的分子。一些结节病患者灌洗细胞中PMN的存在似乎是这种激活的体内生物学关联。这些数据为肺结节病患者BAL细胞活化提供了额外标准，并为吸引炎症细胞进入肺部的因素提供了进一步证据。
