Kahraman Semra, Ulker Kahraman, Pirkevi Çetinkaya Caroline, Kumtepe Colakoglu Yesim, Yelke Hakan K, Yilanlioglu Cihangir
Assisted Reproductive Technologies and Reproductive Genetics Centre, Istanbul Memorial Hospital, Istanbul, Turkey.
Department of Obstetrics and Gynecology, Istanbul Memorial Hospital, Istanbul, Turkey.
SAGE Open Med Case Rep. 2017 Jan 11;5:2050313X16687917. doi: 10.1177/2050313X16687917. eCollection 2017.
To present the first pregnancy achieved in Turkey with frozen-warmed oocytes in a case with previous nine unsuccessful assisted reproductive technology (ART) attempts.
The clinical follow-up of a 33-year-old female applying to our ART centre after a long and complicated history of infertility is described.
In April 2013, the woman attempted our centre for her 10th ART trial. She informed us on oocyte pick-up (OPU) day that her husband had been hospitalized following a car crush in Albania and was unable to travel to our clinic to give a sperm sample. We were therefore placed in the position of having to make an emergency decision. OPU was done and seven oocytes were retrieved. Six metaphase II (MII) oocytes out of seven Cumulus Oocyte Complexes (COCs) were vitrified using the Kitazato Vitrification Cryotop Kit. Six months later, in November 2013, the patient applied for transfer. Two blastocysts were transferred and the ART trial resulted with a singleton pregnancy and the birth of a healthy new-born at term via cesarean section.
Regulation Codes on Assisted Reproductive Procedures and Assisted Reproductive Technology Centres, published in the Official Gazette of the Republic of Turkey, on 6 March 2010 forbade the freezing of gonad cells and tissues except when essential for medical reasons and stated that this would be specified later. However, the Regulation Codes published in the Official Gazette of the Republic of Turkey, on 30 September 2014 provided no further clarification. Unfortunately, the wording of the regulations did not specifically address this unexpected emergency situation. However, we saw our decision to cryopreserve the oocytes as a valid interpretation of the regulations, bearing in mind also the requirement that sperm and oocyte in the IVF process must be those of a married couple. Turkish medicolegal regulations should be revised to increase the chances of more women taking advantage of the procedure to preserve their reproductive autonomy and to give guidance on what action may be taken in emergency situations.
介绍土耳其首例通过冻融卵母细胞实现妊娠的病例，该患者此前9次辅助生殖技术（ART）尝试均未成功。
描述了一名33岁女性在经历漫长而复杂的不孕病史后到我们的ART中心就诊的临床随访情况。
2013年4月，该女性第10次尝试在我们中心进行ART治疗。在取卵日，她告知我们其丈夫在阿尔巴尼亚遭遇车祸后住院，无法前来我们诊所提供精液样本。因此，我们不得不做出紧急决定。进行了取卵操作，获取了7枚卵母细胞。使用Kitazato玻璃化冷冻环试剂盒将7个卵丘卵母细胞复合体（COC）中的6个中期II（MII）卵母细胞进行了玻璃化冷冻。6个月后，即2013年11月，患者前来进行移植。移植了2枚囊胚，此次ART治疗成功实现单胎妊娠，并通过剖宫产足月产下一名健康新生儿。
2010年3月6日在土耳其共和国官方公报上发布的《辅助生殖程序和辅助生殖技术中心管理规范》禁止冷冻性腺细胞和组织，除非出于医学必要，并表示后续将另行规定。然而，2014年9月30日在土耳其共和国官方公报上发布的管理规范并未进一步阐明。遗憾的是，规范的措辞并未具体涉及这种意外的紧急情况。然而，我们认为我们冷冻卵母细胞的决定是对规范的有效解读，同时也牢记体外受精过程中精子和卵母细胞必须来自已婚夫妇这一要求。土耳其的法医学法规应进行修订，以增加更多女性利用该程序维护其生殖自主权的机会，并为紧急情况下可采取的行动提供指导。
