Debenham John J, Tysnes Kristoffer, Khunger Sandhya, Robertson Lucy J
The Norwegian University of Life Sciences (NMBU), Faculty of Veterinary Medicine, Department of Companion Animal Clinical Sciences, Ullevålsveien 72, 0033 Oslo, Norway.
The Norwegian University of Life Sciences (NMBU), Faculty of Veterinary Medicine, Department Food Safety and Infection Biology, Ullevålsveien 72, 0033 Oslo, Norway.
Int J Parasitol Parasites Wildl. 2017 Feb 3;6(1):29-34. doi: 10.1016/j.ijppaw.2016.12.002. eCollection 2017 Apr.
spp., and spp. are intestinal protozoa capable of infecting a range of host species, and are important causes of human morbidity and mortality. Understanding their epidemiology is important, both for public health and for the health of the animals they infect. This study investigated the occurrence of these protozoans in rhesus macaques () in India, with the aim of providing preliminary information on the potential for transmission of these pathogens between macaques and humans. Faecal samples ( = 170) were collected from rhesus macaques from four districts of North-West India. Samples were analysed for using a commercially available direct immunofluorescent antibody test after purification via immunomagnetic separation. Positive samples were characterised by sequencing of PCR products. Occurrence of was investigated first by using a genus-specific PCR, and positive samples further investigated via species-specific PCRs for , , and . cysts were found in 31% of macaque samples, with all isolates belonging to Assemblage B. oocysts were found in 1 sample, however this sample did not result in amplification by PCR. spp. were found in 79% of samples, 49% of which were positive for . Multiplex PCR for , and did not result in amplification in any of the samples. Thus in 51% of the samples positive at the genus specific PCR, the species was not identified. This study provides baseline information on the potential for transmission of these zoonotic parasites at the wildlife-human interface.
某属及某属是能够感染多种宿主物种的肠道原生动物，是导致人类发病和死亡的重要原因。了解它们的流行病学情况，对公共卫生以及它们所感染动物的健康都很重要。本研究调查了印度恒河猴（某物种）中这些原生动物的存在情况，目的是提供有关这些病原体在猕猴与人类之间传播可能性的初步信息。从印度西北部四个地区的恒河猴身上采集了粪便样本（样本数量 = 170）。样本经免疫磁珠分离纯化后，使用市售的直接免疫荧光抗体试验分析某病原体。对阳性样本通过PCR产物测序进行特征分析。首先使用属特异性PCR调查某病原体的存在情况，对阳性样本进一步通过针对某几种特定物种的PCR进行调查。在31%的猕猴样本中发现了某物种的包囊，所有分离株均属于B群。在1个样本中发现了某病原体的卵囊，但该样本通过PCR未扩增出结果。在79%的样本中发现了某属，其中49%对某物种呈阳性。针对某几种特定物种的多重PCR在任何样本中均未扩增出结果。因此，在属特异性PCR呈阳性的样本中，有51%未鉴定出某物种。本研究提供了关于这些人畜共患寄生虫在野生动物与人类界面传播可能性的基线信息。
