Chen C W, Tsai J F, Chuang S E
Institute of Microbiology and Immunology, National Yangming Medical College, Taipei, Taiwan, Republic of China.
Mol Gen Genet. 1987 Aug;209(1):154-8. doi: 10.1007/BF00329851.
An Escherichia coli-Streptomyces shuttle plasmid pIF132 containing two direct mel repeats was constructed. While pIF132 replicated relatively stably in E. coli (Rec+ or recA), its structure was unstable in S. lividans: recombination between the mel repeats resulted in a smaller plasmid, pIF138. Furthermore, pIF132 formed oligomers extensively in E. coli but not in S. lividans.