Barocci S, Nocera A, Carozzi S, Leprini A, Fontana I, Armani R, Valente U, Lamperi S, Celada F
Department of Immunology, XIII USL, Genoa, Italy.
Nephrol Dial Transplant. 1987;2(4):266-70.
Autoreactive lymphocytotoxic antibodies, which have been found in sensitised dialysis patients, are generally not considered to be harmful to a renal allograft. In this work the presence of such autoreactive antibodies was investigated in the following groups of sensitised endstage renal disease patients: (a) dialysis patients waiting for a first kidney transplant, (b) kidney transplanted patients, and (c) dialysis patients with a previous failed graft. Only sera from the above patients which showed high reactivity (greater than 30%) against peripheral blood lymphocytes of a random cell panel (R-PBL), were screened for the presence of autoreactive lymphocytotoxic antibodies, by testing at different temperatures against autologous T lymphoblasts (PHA-ATL) and EBV-induced autologous B lymphoblasts (EBV-ABL). The results showed that both blood transfusions, and viral infections such as cytomegalovirus (CMV), correlated with the presence of autoreactive antibodies, and that in addition, by using PHA-ATL and/or EBV-ABL as absorbing reagents, it was possible to remove the antibodies. These absorption procedures allowed the identification of the presence of autoreactive antibodies alone or in combination with other alloantibodies.
自身反应性淋巴细胞毒性抗体在致敏透析患者中被发现，通常认为对肾移植没有危害。在这项研究中，对以下几组致敏终末期肾病患者自身反应性抗体的存在情况进行了调查：(a)等待首次肾移植的透析患者；(b)肾移植患者；(c)之前移植失败的透析患者。只有上述患者中那些对随机细胞板外周血淋巴细胞（R-PBL）显示高反应性（大于30%）的血清，才通过在不同温度下检测其对自体T淋巴母细胞（PHA-ATL）和EB病毒诱导的自体B淋巴母细胞（EBV-ABL）的反应性，来筛查自身反应性淋巴细胞毒性抗体的存在情况。结果表明，输血以及巨细胞病毒（CMV）等病毒感染均与自身反应性抗体的存在相关，此外，通过使用PHA-ATL和/或EBV-ABL作为吸收试剂，可以去除这些抗体。这些吸收程序能够鉴定自身反应性抗体单独存在或与其他同种抗体共同存在的情况。
