Barocci S, Nocera A, Mossa M, Mantero D, Leprini A, Valente U
Boll Soc Ital Biol Sper. 1989 Aug;65(8):743-50.
A group of 42 sensitized dialysis patients showing high reactivity (81%-100%) against a random panel of peripheral blood lymphocytes (PBL), were analyzed for the presence of autoreactive lymphocytotoxic antibodies. The test was performed at different temperatures (4 degrees C, 22 degrees C, 37 degrees C) with dithiothreitol (DTT) against autologous PBL, EBV-induced autologous B lymphoblasts (A-BCL), K562 cells and T lymphocytes. Thirteen of 42 patients had IgM auto-lymphocytotoxic antibodies. The broadly reactive IgM autoantibodies could be inactivated by treatment with DTT 5 mM and allowed the identification of the presence of autoantibodies alone or in combination with anti-IgG alloantibodies.
对一组42名对随机外周血淋巴细胞（PBL）显示高反应性（81%-100%）的致敏透析患者进行分析，以检测自身反应性淋巴细胞毒性抗体的存在。该测试在不同温度（4℃、22℃、37℃）下，使用二硫苏糖醇（DTT）针对自体PBL、EB病毒诱导的自体B淋巴母细胞（A-BCL）、K562细胞和T淋巴细胞进行。42名患者中有13名具有IgM自身淋巴细胞毒性抗体。广泛反应性的IgM自身抗体可通过用5 mM DTT处理而失活，从而能够单独或与抗IgG同种抗体联合鉴定自身抗体的存在。
