Cetenovic Bojana, Prokic Bogomir, Vasilijic Sasa, Dojcinovic Biljana, Magic Marko, Jokanovic Vukoman, Markovic Dejan
Clinic for Pediatric and Preventive Dentistry, School of Dentistry, University of Belgrade, Belgrade, Serbia.
Department of Surgery, Orthopedy and Ophtalmology, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia.
J Endod. 2017 Mar;43(3):425-432. doi: 10.1016/j.joen.2016.10.041.
The aim of this article was to analyze biocompatibility and bioactivity of new endodontic materials on the basis of nanosynthesized calcium silicates (ALBO-MPCA and ALBO-MPCA) combined with different radiopacifiers in comparison with MTA.
Morphology of the samples was studied by scanning electron microscopy, and the pH and ion release analysis were also assessed. Biocompatibility of materials' eluates (24-hour, 7-day, and 21-day) was conducted by using MTT test. Twelve New Zealand white rabbits were used for intraosseous implantation. Four calvarial defects per animal were created and filled with freshly prepared investigated materials.
Samples mostly consisted of agglomerates built up from nanoparticles, preferably spherical and rod-like. There was no significant difference among pH values of materials' eluates after 24 hours (P > .05). The amount of calcium and aluminum ion release decreased, whereas the amount of magnesium and bismuth (ALBO-MPCA, MTA) and barium (ALBO-MPCA) increased during 21-day period. The metabolic activity of cells increased after the extraction time, except in case of undiluted elutes of ALBO-MPCA and ALBO-MPCA (21-day). Histologic analysis of the samples revealed newly formed bone tissue with moderate inflammation for all investigated materials, which subsided during 90-day period to mild. Both MTA and ALBO-MPCA were in direct contact with the newly formed bone tissue. After 90 days, statistically significant difference in hard tissue formation was observed in comparison of MTA and ALBO-MPCA with control group (P < .05).
Experimental materials ALBO-MPCA and ALBO-MPCA possess both biocompatibility and bioactivity. Because ALBO-MPCA provokes favorable biological response, it is especially good candidate for further clinical investigations.
本文旨在基于纳米合成硅酸钙（ALBO - MPCA和ALBO - MPCA）与不同射线阻射剂结合，对比MTA，分析新型根管治疗材料的生物相容性和生物活性。
通过扫描电子显微镜研究样本形态，并评估pH值和离子释放情况。使用MTT试验对材料洗脱液（24小时、7天和21天）的生物相容性进行检测。选用12只新西兰白兔进行骨内植入。每只动物制造4个颅骨缺损，并用新制备的研究材料填充。
样本大多由纳米颗粒形成的团聚体组成，以球形和棒状为主。24小时后材料洗脱液的pH值之间无显著差异（P＞0.05）。在21天内，钙和铝离子释放量减少，而镁和铋（ALBO - MPCA、MTA）以及钡（ALBO - MPCA）的释放量增加。除了ALBO - MPCA和ALBO - MPCA（21天）的未稀释洗脱液外，提取时间后细胞的代谢活性增加。样本的组织学分析显示，所有研究材料均有新形成的骨组织伴有中度炎症，在90天内炎症消退至轻度。MTA和ALBO - MPCA均与新形成的骨组织直接接触。90天后，与对照组相比，MTA和ALBO - MPCA在硬组织形成方面存在统计学显著差异（P＜0.05）。
实验材料ALBO - MPCA和ALBO - MPCA兼具生物相容性和生物活性。由于ALBO - MPCA引发良好的生物学反应，它是进一步临床研究的特别理想候选材料。
