Vinca Institute of Nuclear Sciences, Mike P. Alasa 12-14, Belgrade, 11001, Serbia.
Institute for Medical Research, Military Medical Academy, Faculty of Medicine, University of Defense, Crnotravska 17, Belgrade, 11000, Serbia.
J Mater Sci Mater Med. 2018 Dec 10;29(12):190. doi: 10.1007/s10856-018-6200-z.
The aim of this study was to investigate the biocompatibility of nanostructured materials based on highly active calcium silicates mixed with different radiocontrast agents in comparison to MTA using in vitro and in vivo model. Morphology of materials' samples was analyzed using SEM while the phase compositions were identified by XRD. pH values of materials' suspensions were conducted by pH-meter. The cytotoxicity of materials' solutions was tested by MTT test (100, 50, 25 and 12.5 mg/ml). LDH and H-thymidine assay were utilized for biocompatibility investigations of materials' eluates (24 h, 7 day and 21 day). Eighteen Guinea pigs were used for intramuscular implantation, as teflon tubes with freshly prepared materials were placed into intramuscular pockets. All samples were composed of round and needle-like particles equally distributed with Ca/Si ratio ~2.7 at%, with the presence of hydrated calcium silicate phases. The pH values of ALBO-MPCA and ALBO-MPCA were high alkaline, while in case of MTA they were lower and continuously declined (p < 0.05). Investigated materials didn't exhibit dose-dependent effect on metabolic activity of L929 cells (p > 0.05). Significant differences in the percentage of cytotoxicity between diluted and undiluted extracts between all tested materials after 24 h and 7 day were noticed (p < 0.05). Increase in L929 cells proliferation was noticed in case of undiluted eluates of ALBO-MPCA and ALBO-MPCA after 7 day (p < 0.05). There were no statistically significant differences in the intensity of inflammatory response between investigated materials and control group after 60 day (p > 0.05). Evaluation of biocompatibility of both ALBO-MPCA and ALBO-MPCA indicate their potential clinical use.
本研究旨在通过体外和体内模型，研究基于高反应性硅酸钙与不同对比剂混合的纳米结构材料的生物相容性，并与 MTA 进行比较。使用 SEM 分析材料样品的形态，使用 XRD 确定相组成。使用 pH 计测量材料悬浮液的 pH 值。通过 MTT 试验（100、50、25 和 12.5 mg/ml）测试材料溶液的细胞毒性。通过 LDH 和 H-胸苷测定法对材料浸出液（24 h、7 天和 21 天）进行生物相容性研究。18 只豚鼠用于肌肉内植入，将新鲜制备的材料放入肌肉内口袋中。所有样品均由均匀分布的圆形和针状颗粒组成，Ca/Si 比约为 2.7%，存在水化硅酸钙相。ALBO-MPCA 和 ALBO-MPCA 的 pH 值呈强碱性，而 MTA 的 pH 值较低且持续下降（p < 0.05）。研究材料对 L929 细胞代谢活性没有剂量依赖性影响（p > 0.05）。在 24 h 和 7 天后，所有测试材料的稀释和未稀释提取物之间的细胞毒性百分比存在显著差异（p < 0.05）。在 7 天后，ALBO-MPCA 和 ALBO-MPCA 的未稀释浸出液中观察到 L929 细胞增殖增加（p < 0.05）。在 60 天后，与对照组相比，研究材料的炎症反应强度没有统计学差异（p > 0.05）。对 ALBO-MPCA 和 ALBO-MPCA 的生物相容性评估表明它们具有潜在的临床应用价值。
