Guardiola F A, Bahi A, Messina C M, Mahdhi A, Santulli A, Arena R, Bakhrouf A, Esteban M A
Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain; Fish Nutrition & Immunobiology Group, Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208, Porto, Portugal.
Laboratory of Analysis, Treatment and Valorization of Pollutants of the Environment and Products, Faculty of Pharmacy, University of Monastir, Tunisia.
Fish Shellfish Immunol. 2017 Apr;63:277-284. doi: 10.1016/j.fsi.2017.02.029. Epub 2017 Feb 21.
The present study was conducted to determine the potential effect of the dietary intake of fenugreek (Trigonella foenum graecum) seeds alone or in combination with Bacillus licheniformis, Lactobacillus plantarum or B. subtilis on gilthead seabream quality and antioxidant response after 2 and 3 weeks of experimental feeding. The results showed that the supplements did not affect the percentage of the fatty acid profiles of muscle, demonstrating that all the additives tested can be administrated without any negative effect on biochemical composition and quality of gilthead seabream. The quantification of thiobarbituric acid reactive substances in muscle demonstrated the significant beneficial effect of the experimental diets compared with the control one. Besides, an increase in superoxide dismutase and catalase in liver was recorded after 3 weeks of administration of experimental diets. Furthermore, real time qPCR revealed that dietary supplementation with FEBS significantly enhances the expression of scavenging enzymes, such as cat and gr genes in the liver after 3 weeks. The findings suggest that the administration of fenugreek supplement alone or combined with probiotic strains could be considered as a good source of natural antioxidants and as a functional aquafeed ingredient for gilthead seabream.
本研究旨在确定单独摄入胡芦巴（Trigonella foenum graecum）种子或与地衣芽孢杆菌、植物乳杆菌或枯草芽孢杆菌联合摄入对实验性投喂2周和3周后金头鲷品质和抗氧化反应的潜在影响。结果表明，这些补充剂不影响肌肉脂肪酸谱的百分比，表明所有测试添加剂的添加不会对金头鲷的生化组成和品质产生任何负面影响。肌肉中硫代巴比妥酸反应性物质的定量分析表明，与对照饲料相比，实验饲料具有显著的有益效果。此外，在投喂实验饲料3周后，肝脏中的超氧化物歧化酶和过氧化氢酶有所增加。此外，实时定量PCR显示，在投喂3周后，添加胡芦巴的饲料显著增强了肝脏中清除酶（如cat和gr基因）的表达。研究结果表明，单独投喂胡芦巴补充剂或与益生菌菌株联合投喂可被视为天然抗氧化剂的良好来源，以及金头鲷功能性水产饲料的成分。
