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编码小鼠和人IgA增强因子及嗜酸性粒细胞集落刺激因子活性的淋巴因子cDNA克隆的分离与特性分析：与白细胞介素5的关系

Isolation and characterization of lymphokine cDNA clones encoding mouse and human IgA-enhancing factor and eosinophil colony-stimulating factor activities: relationship to interleukin 5.

作者信息

Yokota T, Coffman R L, Hagiwara H, Rennick D M, Takebe Y, Yokota K, Gemmell L, Shrader B, Yang G, Meyerson P

机构信息

Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94034-1104.

出版信息

Proc Natl Acad Sci U S A. 1987 Nov;84(21):7388-92. doi: 10.1073/pnas.84.21.7388.

DOI:10.1073/pnas.84.21.7388
PMID:2823259
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC299301/
Abstract

Conditioned medium from the Con A-treated mouse helper T-cell clone Ly1+2-/9 contains activities that enhance the production of IgA by mouse B cells and induce human cord blood cells to form eosinophil colonies. We have isolated a cDNA sequence that expresses IgA-enhancing factor and eosinophil colony-stimulating factor activities from a cDNA library prepared from activated Ly1+2-/9 cells. Based on homology with the mouse cDNA sequence, a human cDNA sequence coding for an interleukin with IgA-enhancing factor and eosinophil colony-stimulating factor activities was isolated from a cDNA library prepared from a human T-cell clone stimulated with anti-T3 antibody and phorbol 12-myristate 13-acetate. DNA sequence analyses revealed that mouse and human cDNA clones encode proteins of 133 and 134 amino acids, respectively, that are identical to cDNA clones encoding the T-cell replacing factor I and B-cell growth factor II activities. These results establish that a single cDNA clone encodes a protein that acts as a growth and differentiation factor for both B cells and eosinophils.

摘要

来自刀豆蛋白A处理的小鼠辅助性T细胞克隆Ly1 + 2 - / 9的条件培养基含有可增强小鼠B细胞IgA产生并诱导人脐血细胞形成嗜酸性粒细胞集落的活性物质。我们从活化的Ly1 + 2 - / 9细胞制备的cDNA文库中分离出了一个表达IgA增强因子和嗜酸性粒细胞集落刺激因子活性的cDNA序列。基于与小鼠cDNA序列的同源性，从用抗T3抗体和佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯刺激的人T细胞克隆制备的cDNA文库中分离出了一个编码具有IgA增强因子和嗜酸性粒细胞集落刺激因子活性的白细胞介素的人cDNA序列。DNA序列分析表明，小鼠和人cDNA克隆分别编码133和134个氨基酸的蛋白质，这些蛋白质与编码T细胞替代因子I和B细胞生长因子II活性的cDNA克隆相同。这些结果表明，单个cDNA克隆编码一种对B细胞和嗜酸性粒细胞均起生长和分化因子作用的蛋白质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d897/299301/3a5658ff37b2/pnas00336-0041-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d897/299301/e82f6013bb48/pnas00336-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d897/299301/46e18503456c/pnas00336-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d897/299301/3879443abfce/pnas00336-0041-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d897/299301/0ac4a1d26c08/pnas00336-0041-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d897/299301/3a5658ff37b2/pnas00336-0041-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d897/299301/e82f6013bb48/pnas00336-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d897/299301/46e18503456c/pnas00336-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d897/299301/3879443abfce/pnas00336-0041-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d897/299301/0ac4a1d26c08/pnas00336-0041-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d897/299301/3a5658ff37b2/pnas00336-0041-d.jpg

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