Lee F, Yokota T, Otsuka T, Meyerson P, Villaret D, Coffman R, Mosmann T, Rennick D, Roehm N, Smith C
Proc Natl Acad Sci U S A. 1986 Apr;83(7):2061-5. doi: 10.1073/pnas.83.7.2061.
A cDNA sequence coding for a unique mouse interleukin that expresses B-cell-, T-cell, and mast-cell-stimulating activities has been isolated from a mouse helper T-cell cDNA library. The library, constructed in the pcD expression vector, was screened by transfecting COS monkey cells with DNA pools to express the products encoded by full-length cDNA inserts. By assaying the transfected cell supernatants, we identified clones encoding a factor that stimulates T-cell and mast cell lines. This factor also induces Ia expression on resting B cells and enhances IgG1 and IgE production by B cells, two properties of B-cell-stimulatory factor 1. The DNA sequence codes for a polypeptide of 140 amino acid residues including a putative signal peptide. These results demonstrate that a single cDNA clone distinct from interleukin 2 and interleukin 3 encodes a polypeptide with multiple biological activities.
从一个小鼠辅助性T细胞cDNA文库中分离出了一个编码独特小鼠白细胞介素的cDNA序列，该白细胞介素具有刺激B细胞、T细胞和肥大细胞的活性。构建于pcD表达载体中的该文库，通过用DNA池转染COS猴细胞以表达全长cDNA插入片段所编码的产物来进行筛选。通过检测转染细胞的上清液，我们鉴定出了编码一种刺激T细胞和肥大细胞系的因子的克隆。该因子还能诱导静止B细胞上Ia的表达，并增强B细胞产生IgG1和IgE的能力，这是B细胞刺激因子1的两个特性。该DNA序列编码一个由140个氨基酸残基组成的多肽，其中包括一个推定的信号肽。这些结果表明，一个不同于白细胞介素2和白细胞介素3的单一cDNA克隆编码了一种具有多种生物学活性的多肽。
