一种编码I型DNA拓扑异构酶的痘苗病毒基因的鉴定。
Identification of a vaccinia virus gene encoding a type I DNA topoisomerase.
作者信息
Shuman S, Moss B
机构信息
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
出版信息
Proc Natl Acad Sci U S A. 1987 Nov;84(21):7478-82. doi: 10.1073/pnas.84.21.7478.
Vaccinia virus encapsidates a type I DNA topoisomerase (EC 5.99.1.2). The enzyme was purified from virus cores to apparent homogeneity, yielding a protein of Mr 32,000. The amino-terminal sequence of the isolated Mr 32,000 polypeptide was determined and used to map the putative structural gene for the vaccinia topoisomerase to the H7r open reading frame of the vaccinia genome. This gene encodes a 314-amino acid polypeptide containing a region homologous to a region of the type I topoisomerase from the yeast Saccharomyces cerevisiae.
痘苗病毒包裹着一种I型DNA拓扑异构酶（EC 5.99.1.2）。该酶从病毒核心中纯化至表观均一，得到一种分子量为32,000的蛋白质。测定了分离出的分子量为32,000的多肽的氨基末端序列，并用于将痘苗拓扑异构酶的推定结构基因定位到痘苗基因组的H7r开放阅读框。该基因编码一个314个氨基酸的多肽，其中包含一个与酿酒酵母I型拓扑异构酶的一个区域同源的区域。
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