Gao Yi, Qin Lihong, Yang Yuwei, Dong Xue, Zhao Zijiao, Zhang Guoliang, Zhao Zhihui
Branch of Animal Husbandry, Jilin Academy of Agricultural Science, Gongzhuling, Jilin 136100, China; College of Animal Science and Technology, Jilin Agriculture University, Xin Cheng Street 2888, Changchun, Jilin 130118, China.
Branch of Animal Husbandry, Jilin Academy of Agricultural Science, Gongzhuling, Jilin 136100, China.
Anim Reprod Sci. 2017 Apr;179:35-43. doi: 10.1016/j.anireprosci.2017.01.014. Epub 2017 Feb 4.
Podoplanin (PDPN) is a transmembrane receptor which is involved in various physiological and pathological processes, such as cell motility, invasion, tumor metastasis and blood vessels formation. Although there are reports on the involvement of PDPN in Sertoli cells in human and mice, the role of PDPN on the development of bovine Sertoli cells has not been reported. In the present study, Sertoli cells were isolated from 1-day-old bovine testes by two steps enzyme digestion method. Feulgen staining of satellite karyosomes and inhibin immunofluorescence staining suggested that the isolated immature Sertoli cells were very pure. Transfection with overexpression plasmid pBI-CMV3-PDPN and interference shRNA plasmid indicated that PDPN could significantly promote Sertoli cells cycle progression, cells proliferation and androgen-binding protein (ABP) production. Our results indicated that PDPN gene plays a significant role in the proliferation and maturation of bovine Sertoli cells.
血小板源性生长因子结合蛋白（Podoplanin，PDPN）是一种跨膜受体，参与多种生理和病理过程，如细胞运动、侵袭、肿瘤转移和血管形成。尽管有关于PDPN在人和小鼠支持细胞中作用的报道，但PDPN在牛支持细胞发育中的作用尚未见报道。在本研究中，采用两步酶消化法从1日龄牛睾丸中分离支持细胞。卫星核仁的福尔根染色和抑制素免疫荧光染色表明，分离得到的未成熟支持细胞纯度很高。用过表达质粒pBI-CMV3-PDPN和干扰shRNA质粒转染表明，PDPN可显著促进支持细胞周期进程、细胞增殖和雄激素结合蛋白（ABP）的产生。我们的结果表明，PDPN基因在牛支持细胞的增殖和成熟中起重要作用。
