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中国草原红牛未成熟与成熟睾丸组织间miRNA与靶mRNA的综合分析

Comprehensive Analysis of miRNAs and Target mRNAs between Immature and Mature Testis Tissue in Chinese Red Steppes Cattle.

作者信息

Fang Xibi, Qin Lihong, Yu Haibin, Jiang Ping, Xia Lixin, Gao Zhen, Yang Runjun, Zhao Yumin, Yu Xianzhong, Zhao Zhihui

机构信息

College of Animal Science, Jilin University, Changchun 130062, China.

Branch of Animal Husbandry, Jilin Academy of Agricultural Sciences, Changchun 130033, China.

出版信息

Animals (Basel). 2021 Oct 21;11(11):3024. doi: 10.3390/ani11113024.

DOI:10.3390/ani11113024
PMID:34827757
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8614260/
Abstract

This study aims to screen potential regulators and regulate fecundity networks between microRNAs (miRNAs) and target genes. The bovine testes of immature and mature Chinese Red Steppes were performed by genome-wide analysis of mRNAs and miRNAs. Compared with testicular tissues of newborns, 6051 upregulated genes and 7104 downregulated genes in adult cattle were identified as differentially expressed genes (DEGs). The DEGs were significantly enriched in 808 GO terms ( < 0.05) including male gonad development, male genitalia development, spermatogenesis, and sperm motility. Moreover, DEGs were also significantly enriched in 105 KEGG pathways ( < 0.05), including cGMP-PKG signaling pathway and calcium signaling pathway. To explore the expression of miRNA-regulated gene expression, 896 differentially expressed target genes negatively regulated with the expression levels of 31 differentially expressed miRNAs (DERs) were predicted and analyzed, and a network-integrated analysis was constructed. Furthermore, real-time PCR was performed to verify the expression levels of DEGs and DERs. Our results identified novel candidate DEGs and DERs correlated with male reproduction and intricate regulating networks between miRNAs and genes, which will be valuable for future genetic and epigenetic studies of sperm development and maturity, as well as providing valuable insights into the molecular mechanisms of male fertility and spermatogenesis in cattle.

摘要

本研究旨在筛选潜在的调控因子，并调节微小RNA（miRNA）与靶基因之间的繁殖力网络。对中国草原红牛未成熟和成熟公牛的睾丸进行了mRNA和miRNA的全基因组分析。与新生牛的睾丸组织相比，成年牛中有6051个上调基因和7104个下调基因被鉴定为差异表达基因（DEG）。这些DEG在808个GO术语（<0.05）中显著富集，包括雄性性腺发育、雄性生殖器发育、精子发生和精子活力。此外，DEG在105条KEGG通路（<0.05）中也显著富集，包括cGMP-PKG信号通路和钙信号通路。为了探索miRNA调控基因表达的情况，预测并分析了896个与31个差异表达miRNA（DER）表达水平呈负调控的差异表达靶基因，并构建了网络整合分析。此外，进行实时PCR以验证DEG和DER的表达水平。我们的结果鉴定出了与雄性繁殖相关的新的候选DEG和DER，以及miRNA与基因之间复杂的调控网络，这将对未来精子发育和成熟的遗传和表观遗传学研究具有重要价值，也为牛雄性生育力和精子发生的分子机制提供了有价值的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fc/8614260/b8a554591619/animals-11-03024-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fc/8614260/3381b0dee640/animals-11-03024-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fc/8614260/773a32ed2351/animals-11-03024-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fc/8614260/2162bc27ddfc/animals-11-03024-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fc/8614260/a1a6d96b1b48/animals-11-03024-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fc/8614260/04d07c5484ab/animals-11-03024-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fc/8614260/b8a554591619/animals-11-03024-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fc/8614260/3381b0dee640/animals-11-03024-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fc/8614260/773a32ed2351/animals-11-03024-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fc/8614260/2162bc27ddfc/animals-11-03024-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fc/8614260/a1a6d96b1b48/animals-11-03024-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fc/8614260/04d07c5484ab/animals-11-03024-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91fc/8614260/b8a554591619/animals-11-03024-g006.jpg

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