Higgins A J, Lees P, Sedgwick A D
Animal Health Trust, Newmarket, Suffolk.
Vet Rec. 1987 May 30;120(22):517-22. doi: 10.1136/vr.120.22.517.
Two experimental models of acute non-immune inflammation have been developed to enable studies of the biochemical composition and cellular content of exudates to be undertaken. Both are based on the creation of a mild, reproducible and reversible inflammatory reaction, which is free from uncontrolled incidental factors and which causes minimal distress to the experimental animals. The polyester sponge model involves the insertion of small polyester sponge strips soaked in sterile carrageenan solution into subcutaneous neck pouches and their serial removal. The tissue-cage model is based on the initial insertion of a spherical tissue-cage subcutaneously in the neck and the subsequent stimulation with carrageenan of the granulation tissue which lines and permeates the cage. The acute inflammatory exudates have been shown to contain eicosanoids with prostaglandin E2 predominant. Polymorphonuclear leucocyte numbers increased progressively in the polyester sponge model, whereas cell numbers were maximal at 12 hours in the tissue-cage model. The relationships between eicosanoid formation at the site of inflammation and leucocyte accumulation, enzyme release, total protein content of exudates and the temperature of the lesions have been investigated.
已开发出两种急性非免疫性炎症实验模型，以便能够对渗出液的生化成分和细胞成分进行研究。这两种模型均基于产生轻度、可重复且可逆的炎症反应，该反应不受 uncontrolled incidental factors 影响，并且对实验动物造成的痛苦最小。聚酯海绵模型包括将浸泡在无菌角叉菜胶溶液中的小聚酯海绵条插入颈部皮下袋囊中，并对其进行连续移除。组织笼模型基于首先将球形组织笼皮下植入颈部，随后用角叉菜胶刺激衬于笼内并渗透笼体的肉芽组织。急性炎症渗出液已被证明含有以前列腺素 E2 为主的类花生酸。在聚酯海绵模型中，多形核白细胞数量逐渐增加，而在组织笼模型中，细胞数量在 12 小时时达到最大值。已对炎症部位类花生酸形成与白细胞聚集、酶释放、渗出液总蛋白含量以及损伤部位温度之间的关系进行了研究。
