Guthrie A J, Short C R, Swan G E, Mülders M S, Killeen V M, Nurton J P
Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
J Vet Pharmacol Ther. 1996 Feb;19(1):44-9. doi: 10.1111/j.1365-2885.1996.tb00007.x.
This paper describes the use of subcutaneously-placed tissue chambers as a sterile soft-tissue inflammation model in Thoroughbred horses. Acute, non-immune inflammation was initiated by injecting a sterile lambda carrageenan solution into a tissue chamber. This model was used to study the temporal changes in oxygen and carbon dioxide tensions, pH, bicarbonate, protein, albumin, prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) concentrations, cell counts and differential counts in tissue fluid from inflamed tissue chambers and control chambers. Skin temperatures over control and inflamed chambers were also compared. Carrageenan-induced inflammation resulted in significant increases in tissue-fluid carbon dioxide tension, leucocyte count, albumin, and PGE2 and LTB4 concentrations. It also resulted in a significant decrease in tissue fluid pH and HCO3-concentration. Inflammation did not result in significant changes in tissue-fluid protein concentration, differential cell counts or skin temperature over the chambers. The use of this type of tissue chamber is well-suited for studying the pathophysiology of a self-contained, non-immune inflammatory process. The model described in this paper could prove to be very useful in studies of the distribution of anti-inflammatory drugs and the effects of such drugs on various aspects of the inflammatory process.
本文描述了将皮下放置的组织腔作为纯种马无菌软组织炎症模型的应用。通过向组织腔内注射无菌λ-角叉菜胶溶液引发急性非免疫性炎症。该模型用于研究炎症组织腔和对照组织腔组织液中氧和二氧化碳张力、pH值、碳酸氢盐、蛋白质、白蛋白、前列腺素E2（PGE2）和白三烯B4（LTB4）浓度、细胞计数和分类计数的时间变化。还比较了对照腔和炎症腔上方的皮肤温度。角叉菜胶诱导的炎症导致组织液二氧化碳张力、白细胞计数、白蛋白以及PGE2和LTB4浓度显著增加。它还导致组织液pH值和HCO3浓度显著降低。炎症并未导致组织液蛋白质浓度、细胞分类计数或腔上方皮肤温度发生显著变化。这种类型的组织腔非常适合用于研究自限性非免疫性炎症过程的病理生理学。本文所述模型在抗炎药物分布研究以及此类药物对炎症过程各个方面的影响研究中可能非常有用。
