Godeny E K, Gauntt C J
Wistar Institute, Philadelphia, Pennsylvania.
Am J Pathol. 1987 Nov;129(2):267-76.
In adolescent CD-1 male mice inoculated with a myocarditic coxsackievirus B3 (CVB3m) acute focal lesions containing necrotic myocytes, infiltrating mononuclear cells, and fibroblasts develop. With the use of an in situ immune autoradiographic method with rat monoclonal antibodies (MAb) and an 35S-labeled antibody, viral antigens were detected outside of lesions. Macrophages, T lymphocytes, and natural killer (NK) cells were identified within myocarditic lesions during the acute phase of the disease. Macrophages detected by anti-Mac-1 MAb were in focal areas within myocarditic lesions on Days 4-7 after inoculation. T lymphocytes were detected in myocarditic lesions on Days 4-10, with MAb to Thy-1 and Lyt-1 antigens showing diffuse reaction patterns, suggesting random distribution of these cells in lesions. Focal areas of reactivity were detected with MAbs to L3T4 and Lyt-2 antigens, suggesting clusters of helper and cytotoxic/suppressor T lymphocytes, respectively. NK cells were presumptively detected by asialo GM1 surface marker in lesions at all times. The presence of activated NK cells in lesions was confirmed by assay of mechanically dissociated heart tissues on Day 8. These data describe the temporal sequence and identity of leukocytes entering into CVB3-induced focal myocarditic lesions during the acute phase of disease in CD-1 mice.
在接种了致心肌炎柯萨奇病毒B3（CVB3m）的青春期CD-1雄性小鼠中，出现了含有坏死心肌细胞、浸润的单核细胞和成纤维细胞的急性局灶性病变。使用大鼠单克隆抗体（MAb）和35S标记抗体的原位免疫放射自显影方法，在病变外检测到病毒抗原。在疾病急性期，在心肌炎病变中鉴定出巨噬细胞、T淋巴细胞和自然杀伤（NK）细胞。接种后第4至7天，用抗Mac-1单克隆抗体检测到的巨噬细胞位于心肌炎病变的局灶区域。在第4至10天，在心肌炎病变中检测到T淋巴细胞，针对Thy-1和Lyt-1抗原的单克隆抗体显示出弥漫性反应模式，表明这些细胞在病变中随机分布。用针对L3T4和Lyt-2抗原的单克隆抗体检测到反应性局灶区域，分别表明存在辅助性T淋巴细胞和细胞毒性/抑制性T淋巴细胞簇。在病变中始终通过无唾液酸GM1表面标志物推测性地检测到NK细胞。在第8天通过对机械解离的心脏组织进行检测，证实了病变中存在活化的NK细胞。这些数据描述了在CD-1小鼠疾病急性期，白细胞进入CVB3诱导的局灶性心肌炎病变的时间顺序和特征。
