Li Haoyang, Wang Sheng, Lǚ Kai, Yin Bin, Xiao Bang, Li Sedong, He Jianguo, Li Chaozheng
State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, China.
FEBS Lett. 2017 Apr;591(7):1010-1017. doi: 10.1002/1873-3468.12607. Epub 2017 Mar 16.
It has been proposed that invertebrate stimulators of interferon genes (STINGs) do not take part in the innate immune response to infection. Herein, we identified a new STING homolog from pacific white shrimp Litopenaeus vannamei (LvSTING). Some amino acids crucial for recognizing cyclic dinucleotides in mammals are highly conserved in LvSTING. Moreover, LvSTING expression can be robustly induced by challenge with the Gram-negative bacteria Vibrio parahaemolyticus. Silencing of LvSTING contributes to decreased expression of the antimicrobial peptide PEN4 and renders shrimp more susceptible to V. parahaemolyticus infection, while coinjection with the recombinant LvSTING protein can rescue PEN4 expression in vivo and confer shrimp with more resistance to infection. Taken together, these results suggest that LvSTING is involved in the innate immune response to bacterial infection.
有人提出，无脊椎动物的干扰素基因刺激因子（STINGs）不参与对感染的先天免疫反应。在此，我们从凡纳滨对虾（Litopenaeus vannamei）中鉴定出一种新的STING同源物（LvSTING）。在哺乳动物中对识别环状二核苷酸至关重要的一些氨基酸在LvSTING中高度保守。此外，用革兰氏阴性菌副溶血性弧菌攻击可强烈诱导LvSTING表达。沉默LvSTING会导致抗菌肽PEN4表达降低，并使虾更容易受到副溶血性弧菌感染，而与重组LvSTING蛋白共同注射可在体内挽救PEN4表达，并使虾对感染具有更强的抵抗力。综上所述，这些结果表明LvSTING参与了对细菌感染的先天免疫反应。
