A Glutathione Peroxidase Gene from Is Involved in Oxidative Stress Responses and Pathogen Infection Resistance.

In shrimp, several glutathione peroxidase (GPX) genes have been cloned and functionally studied. Increasing evidence suggests the genes' involvement in white spot syndrome virus (WSSV)- or -infection resistance. In the present study, a novel GXP gene () was cloned in . Promoter of was activated by NF-E2-related factor 2. Further study showed that expression was evidently accelerated by oxidative stress or WSSV or infection. Consistently, downregulated expression of increased the cumulative mortality of WSSV- or -infected shrimp. Similar results occurred in shrimp suffering from oxidative stress. Moreover, LvGPX3 was important for enhancing () gene expression in S2 cells with lipopolysaccharide treatment. Further, knockdown of expression significantly suppressed expression of AMPs, such as , and in shrimp. AMPs have been proven to be engaged in shrimp WSSV- or -infection resistance; it was inferred that LvGPX3 might enhance shrimp immune response under immune challenges, such as increasing expression of AMPs. The regulation mechanism remains to be further studied.