Key Laboratory for Healthy and Safe Aquaculture/Institute of Modern Aquaculture Science and Engineering (IMASE), College of Life Science, South China Normal University, Guangzhou 510631, China.
Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China.
Int J Mol Sci. 2022 Jan 5;23(1):567. doi: 10.3390/ijms23010567.
In shrimp, several glutathione peroxidase (GPX) genes have been cloned and functionally studied. Increasing evidence suggests the genes' involvement in white spot syndrome virus (WSSV)- or -infection resistance. In the present study, a novel GXP gene () was cloned in . Promoter of was activated by NF-E2-related factor 2. Further study showed that expression was evidently accelerated by oxidative stress or WSSV or infection. Consistently, downregulated expression of increased the cumulative mortality of WSSV- or -infected shrimp. Similar results occurred in shrimp suffering from oxidative stress. Moreover, LvGPX3 was important for enhancing () gene expression in S2 cells with lipopolysaccharide treatment. Further, knockdown of expression significantly suppressed expression of AMPs, such as , and in shrimp. AMPs have been proven to be engaged in shrimp WSSV- or -infection resistance; it was inferred that LvGPX3 might enhance shrimp immune response under immune challenges, such as increasing expression of AMPs. The regulation mechanism remains to be further studied.
在虾中，已经克隆并研究了几种谷胱甘肽过氧化物酶 (GPX) 基因。越来越多的证据表明，这些基因参与了白斑综合征病毒 (WSSV) 或感染的抗性。在本研究中，在 中克隆了一个新的 GXP 基因 ()。 的启动子被 NF-E2 相关因子 2 激活。进一步的研究表明，氧化应激或 WSSV 或 感染明显加速了 的表达。一致地， 的下调表达增加了 WSSV 或感染的虾的累积死亡率。在遭受氧化应激的虾中也出现了类似的结果。此外，LvGPX3 对于增强 LPS 处理的 S2 细胞中的 ()基因表达很重要。此外， 表达的敲低显著抑制了虾中 AMPs 的表达，如 、 和 。AMP 已被证明参与虾的 WSSV 或感染抗性；可以推断，LvGPX3 可能在免疫挑战下增强虾的免疫反应，例如增加 AMPs 的表达。调节机制仍有待进一步研究。
