Zellen G K, Thorsen J
Ontario Ministry of Agriculture and Food, Veterinary Services Laboratory, Guelph, Canada.
Avian Dis. 1987 Jul-Sep;31(3):455-8.
The indirect enzyme-linked immunosorbent assay (ELISA) was applied to detect antibody to infectious bronchitis (IB) virus in chickens. A cross-reactivity study was performed to determine the antigenic relationships among six strains: Massachusetts, Connecticut, Beaudette, and three Ontario field isolates. For comparison, the cross-reactivity study was performed in eggs using the serum-neutralization (SN) test for IB. The ELISA proved to be more broadly cross-reactive than the IB-SN test. This suggests that the ELISA for IB will be useful as a rapid diagnostic test for serum profiling in unvaccinated flocks, regardless of the IB serotype involved.
采用间接酶联免疫吸附测定法（ELISA）检测鸡传染性支气管炎（IB）病毒抗体。进行了交叉反应性研究，以确定六种毒株之间的抗原关系，这六种毒株分别是：马萨诸塞毒株、康涅狄格毒株、博德特毒株以及三个安大略省田间分离株。为作比较，在鸡蛋中采用IB血清中和试验（SN）进行交叉反应性研究。结果证明，ELISA的交叉反应性比IB-SN试验更广泛。这表明，无论涉及何种IB血清型，用于IB检测的ELISA作为一种针对未接种疫苗鸡群血清分析的快速诊断试验将很有用。
