Cowen B S, Hitchner S B
Avian Dis. 1975 Jul-Sep;19(3):583-95.
The antigenic relationships of 12 strains of infectious bronchitis virus (IBV) were evaluated by a virus-neutralization procedure similar to that used in typing human rhinoviruses. Such a procedure consists of reciprocal neutralization tests performed by reacting 32-320 EID50 or plaque-forming units of virus with 20 antibody units of antiserum. Eight serologic groups were identified by chicken embryo assay, and 4 by plaque-reduction (90%). In general, serologic groupings were not distinct but reflected numerous intergroup relationships. The contrasting results exhibited by indicator systems are viewed as differences in the accuracy of the methods employed. It is suggested that before an IBV classification scheme can be proposed, agreement must be reached on the most suitable indicator system, techniques must be standardized, and reference viruses and antisera distributed to several institutions for comparison testing. In addition, cross-protection studies are needed to determine the importance of IBV serotypes and/or variants on vaccine efficacy.
采用一种类似于对人鼻病毒进行分型时所用的病毒中和程序，对12株传染性支气管炎病毒（IBV）的抗原关系进行了评估。该程序包括通过使32 - 320个半数鸡胚感染量（EID50）或空斑形成单位的病毒与20个抗体单位的抗血清反应来进行双向中和试验。通过鸡胚试验鉴定出8个血清学组，通过空斑减少（90%）鉴定出4个血清学组。一般来说，血清学分组并不明显，而是反映出众多组间关系。指示系统所呈现的对比结果被视为所用方法准确性的差异。建议在提出IBV分类方案之前，必须就最合适的指示系统达成一致，技术必须标准化，并且将参考病毒和抗血清分发给多个机构进行比较测试。此外，需要进行交叉保护研究以确定IBV血清型和/或变异体对疫苗效力的重要性。
