López José I, Cortés Jesús M
Department of Pathology, Cruces University Hospital, 48903 Barakaldo, Spain; Biomarkers in Cancer Unit, Biocruces Research Institute, 48903 Barakaldo, Spain; University of the Basque Country (UPV/EHU), 48940 Leioa, Spain.
Quantitative Biomedicine Unit, Biocruces Research Institute, 48903 Barakaldo, Spain; Ikerbasque: The Basque Foundation for Science, 48013 Bilbao, Spain; Department of Cell Biology and Histology, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain.
Hum Pathol. 2017 Jun;64:1-6. doi: 10.1016/j.humpath.2017.02.010. Epub 2017 Feb 23.
Intratumor heterogeneity (ITH) is increasingly being recognized as a highly complex process with high clinical impact that deserves special attention from practicing pathologists. The value of the ITH detection depends on the correctness of the pathologist's sampling. The goal of this review is 2-fold. On the one hand, we provide a basic scientific context for the practical pathologist's perspective. On the other hand, we encourage pathologists to adopt a more scientific and up-to-date approach to a key component of their daily work, namely, how to sample a tumor for reliable histological and molecular analysis. In particular, we review the consecutive steps of an efficient alternative to traditional approaches for detecting ITH: multisite tumor sampling. Notably, this type of sampling, based on a divide-and-conquer algorithm, is supported by scientific evidence showing its clinical applicability and practical advantages at no extra cost.
肿瘤内异质性（ITH）日益被认为是一个高度复杂的过程，具有重大临床影响，值得执业病理学家特别关注。ITH检测的价值取决于病理学家采样的正确性。本综述的目标有两个。一方面，我们从实际病理学家的角度提供一个基础科学背景。另一方面，我们鼓励病理学家对其日常工作的一个关键组成部分采用更科学、更新的方法，即如何对肿瘤进行采样以进行可靠的组织学和分子分析。特别是，我们回顾了一种高效替代传统ITH检测方法的连续步骤：多部位肿瘤采样。值得注意的是，这种基于分治算法的采样方式有科学证据支持，表明其具有临床适用性且无需额外成本，具有实际优势。
