Pioltine Marina B, de Melo Maria Edna, Santos Aritânia, Machado Alisson D, Fernandes Ariana E, Fujiwara Clarissa T, Cercato Cintia, Mancini Marcio C
Hospital das Clinicasy, Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
J Nutrigenet Nutrigenomics. 2016;9(5-6):300-305. doi: 10.1159/000455915. Epub 2017 Feb 25.
BACKGROUND/AIMS: Taste is recognized as an important predictor of food choices. Thus, polymorphisms in genes encoding taste receptors may explain the variability in food preference and intake. Here, we aimed to determine whether genetic variation in the CD36 gene affects food intake and risk of obesity.
A cross-sectional study was conducted with obese Brazilian children and adolescents (n = 466; BMI-for-age z-score [zBMI] 3.29 ± 0.61) and normal-weight children (n = 114; zBMI -0.11 ± 0.7). To assess the obesity risk according to genotypes, a logistic regression adjusted for age and gender was performed. Two 24-h food recalls assessed total energy (kcal/day) and macronutrient (% kcal and g/day) intake, consumption of sweet and fatty tasting foods (portion and g/day), as well as the most commonly consumed foods (mL or g/day). The food portion sizes were measured according to Brazilian guidelines. The genetic variant rs1761667 (A/G) in CD36 was genotyped by real-time PCR.
We found no relationship between rs1761667 genotypes and obesity risk. A significant genetic association between CD36 genotype and fat intake was observed for the A allele of rs1761667, which was associated with a decreased intake of total fat (g/day) (p = 0.01), polyunsaturated and monounsaturated fatty acids (% kcal and g/day), total sugars (g/day) (p = 0.01), fatty foods (portion and g/day) (p < 0.001 for both), and vegetable oils (mL/day) (p = 0.02) only in obese subjects. No differences were found between normal-weight children.
The A allele of the rs1761667 single nucleotide polymorphism in CD36 is associated with decreased fat and sugar intake in obese children and adolescents.
背景/目的：味觉被认为是食物选择的重要预测指标。因此，编码味觉受体的基因多态性可能解释食物偏好和摄入量的差异。在此，我们旨在确定CD36基因的遗传变异是否会影响食物摄入量和肥胖风险。
对肥胖的巴西儿童和青少年（n = 466；年龄别BMI z评分[zBMI] 3.29±0.61）和正常体重儿童（n = 114；zBMI -0.11±0.7）进行了一项横断面研究。为了根据基因型评估肥胖风险，进行了一项针对年龄和性别的逻辑回归分析。通过两次24小时食物回忆法评估总能量（千卡/天）和宏量营养素（千卡百分比和克/天）摄入量、甜味和高脂肪口味食物的消费量（份和克/天）以及最常食用的食物（毫升或克/天）。食物份量根据巴西指南进行测量。通过实时PCR对CD36基因中的rs1761667（A/G）基因变异进行基因分型。
我们发现rs1761667基因型与肥胖风险之间没有关系。观察到rs1761667的A等位基因与CD36基因型和脂肪摄入量之间存在显著的遗传关联，仅在肥胖受试者中，该等位基因与总脂肪摄入量（克/天）降低（p = 0.01）、多不饱和脂肪酸和单不饱和脂肪酸（千卡百分比和克/天）、总糖（克/天）（p = 0.01）、高脂肪食物（份和克/天）（两者均p < 千分之一）以及植物油（毫升/天）（p = 0.02）有关。正常体重儿童之间未发现差异。
CD36基因中rs1761667单核苷酸多态性的A等位基因与肥胖儿童和青少年脂肪和糖摄入量降低有关。
