Kobayashi Masanori, Saito Akiko, Tanaka Yoshikazu, Michishita Masaki, Kobayashi Masato, Irimajiri Mami, Kaneda Takeharu, Ochiai Kazuhiko, Bonkobara Makoto, Takahashi Kimimasa, Hori Tatsuya, Kawakami Eiichi
Laboratory of Reproduction, Division of Therapeutic Science II, Department of Clinical Veterinary Medicine, School of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan.
J Vet Med Sci. 2017 Apr 5;79(4):719-725. doi: 10.1292/jvms.16-0279. Epub 2017 Feb 27.
Canine prostate cancer (cPCa) is an untreatable malignant neoplasm resulting in local tissue invasion and distant metastasis. MicroRNAs (miRs) are small non-coding RNAs that function as oncogenes or tumor suppressors. The purpose of this study was to characterize the expression of miRs that are altered in cPCa tissue. The expression levels of 277 mature miRs in prostatic tissue (n=5, respectively) were compared between the non-tumor and tumor groups using real-time PCR. Five miRs (miR-18a, 95, 221, 222 and 330) were up-regulated, but 14 miRs (miR-127, 148a, 205, 299, 329b, 335, 376a, 376c, 379, 380, 381, 411, 487b and 495) were down-regulated specifically in cPCa (P<0.05). These miRs have potential use as early diagnosis markers for cPCa and in miR-based therapy.
犬前列腺癌（cPCa）是一种无法治愈的恶性肿瘤，可导致局部组织浸润和远处转移。微小RNA（miRs）是一类小的非编码RNA，可作为癌基因或肿瘤抑制因子发挥作用。本研究的目的是表征在cPCa组织中发生改变的miRs的表达情况。使用实时PCR比较了非肿瘤组和肿瘤组前列腺组织（各n = 5）中277种成熟miRs的表达水平。5种miRs（miR-18a、95、221、222和330）上调，但14种miRs（miR-127、148a、205、299、329b、335、376a、376c, 379, 380, 381, 411, 487b和495）在cPCa中特异性下调（P<0.05）。这些miRs有潜力用作cPCa的早期诊断标志物以及基于miR的治疗。
