Artem'ev N O, Garnovskaia M n, Dumler I L, Etingof R N
Biokhimiia. 1987 Sep;52(9):1438-44.
The soluble fraction of uterine tissue was found to contain a factor which is a potent activator of various cyclic nucleotide phosphodiesterases (with the exception of the retinal photoreceptor cell enzyme). The protein origin of this factor was established, using proteolysis, precipitation with trichloroacetic acid. The activator was purified by gel filtration on Sephadex G-25 and Biogel P-4 as well as by a highly effective liquid chromatography. The activator was shown to be a peptide with Mr = 1150 Da. The peptide was thermostable and stable within a broad range of pH. Besides phosphodiesterases, the peptide activated 5'-nucleotidase and Mg2+-ATPase of photoreceptor membranes.
研究发现，子宫组织的可溶部分含有一种因子，它是各种环核苷酸磷酸二酯酶（视网膜光感受器细胞酶除外）的强效激活剂。利用蛋白水解、三氯乙酸沉淀确定了该因子的蛋白质来源。通过在Sephadex G-25和Biogel P-4上进行凝胶过滤以及高效液相色谱法对激活剂进行了纯化。结果表明，该激活剂是一种分子量为1150 Da的肽。该肽具有热稳定性，并且在很宽的pH范围内都稳定。除了磷酸二酯酶外，该肽还能激活光感受器膜的5'-核苷酸酶和Mg2+ -ATP酶。
