Novikov I A, Vakhrushev I V, Antonov E N, Yarygin K N, Subbot A M
Institute of Eye Diseases, Moscow, Russia.
V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
Bull Exp Biol Med. 2017 Feb;162(4):558-562. doi: 10.1007/s10517-017-3659-4. Epub 2017 Feb 27.
Mesenchymal stromal cells from deciduous teeth in 2D- and 3D-cultures on culture plastic, silicate glass, porous polystyrene, and experimental polylactoglycolide matrices were visualized by scanning electron microscopy with lanthanide contrasting. Supravital staining of cell cultures with a lanthanide-based dye (neodymium chloride) preserved normal cell morphology and allowed assessment of the matrix properties of the carriers. The developed approach can be used for the development of biomaterials for tissue engineering.
通过镧系元素对比扫描电子显微镜对在培养塑料、硅酸盐玻璃、多孔聚苯乙烯和实验性聚乳酸乙醇酸酯基质上进行二维和三维培养的乳牙间充质基质细胞进行了可视化观察。用基于镧系元素的染料（氯化钕）对细胞培养物进行超活染色可保持细胞正常形态，并能够评估载体的基质特性。所开发的方法可用于组织工程生物材料的研发。
