Braghirolli Daikelly Iglesias, Zamboni Fernanda, Acasigua Gerson A X, Pranke Patricia
Hematology and Stem Cells Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil ; Department of Materials Science, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Hematology and Stem Cells Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Int J Nanomedicine. 2015 Aug 14;10:5159-69. doi: 10.2147/IJN.S84312. eCollection 2015.
In tissue engineering, a uniform cell occupation of scaffolds is crucial to ensure the success of tissue regeneration. However, this point remains an unsolved problem in 3D scaffolds. In this study, a direct method to integrate cells into fiber scaffolds was investigated by combining the methods of electrospinning of fibers and bioelectrospraying of cells. With the associating of these methods, the cells were incorporated into the 3D scaffolds while the fibers were being produced. The scaffolds containing cells (SCCs) were produced using 20% poly(lactide-co-glycolide) solution for electrospinning and mesenchymal stem cells from deciduous teeth as a suspension for bioelectrospraying. After their production, the SCCs were cultivated for 15 days at 37°C with an atmosphere of 5% CO2. The 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide test demonstrated that the cells remained viable and were able to grow between the fibers. Scanning electron microscopy showed the presence of a high number of cells in the structure of the scaffolds and confocal images demonstrated that the cells were able to adapt and spread between the fibers. Histological analysis of the SCCs after 1 day of cultivation showed that the cells were uniformly distributed throughout the thickness of the scaffolds. Some physicochemical properties of the scaffolds were also investigated. SCCs exhibited good mechanical properties, compatible with their handling and further implantation. The results obtained in the present study suggest that the association of electrospinning and bioelectrospraying provides an interesting tool for forming 3D cell-integrated scaffolds, making it a viable alternative for use in tissue engineering.
在组织工程中，支架材料中细胞的均匀分布对于确保组织再生的成功至关重要。然而，这一点在三维支架中仍是一个未解决的问题。在本研究中，通过结合纤维静电纺丝和细胞生物电喷雾的方法，研究了一种将细胞整合到纤维支架中的直接方法。通过这些方法的结合，在生产纤维的同时将细胞整合到三维支架中。使用20%的聚（丙交酯-共-乙交酯）溶液进行静电纺丝，并将乳牙间充质干细胞作为悬浮液进行生物电喷雾，制备了含细胞支架（SCCs）。制备完成后，将SCCs在37°C、5%二氧化碳气氛中培养15天。3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐试验表明，细胞保持活力并能够在纤维间生长。扫描电子显微镜显示支架结构中有大量细胞存在，共聚焦图像表明细胞能够在纤维间适应并扩散。培养1天后对SCCs进行组织学分析，结果显示细胞在支架的整个厚度上均匀分布。还研究了支架的一些物理化学性质。SCCs表现出良好的力学性能，便于操作和进一步植入。本研究获得的结果表明，静电纺丝和生物电喷雾相结合为形成三维细胞整合支架提供了一种有趣的工具，使其成为组织工程中一种可行的替代方法。
