Wesemann W, Rotsch M, Schulz E, Zöfel P
Abteilung Neurochemie, Philipps-Universität, Marburg, F.R.G.
Chronobiol Int. 1986;3(2):141-6. doi: 10.3109/07420528609066359.
Sleep deprivation (SD) modified the circadian rhythm of specific high affinity serotonin (5-HT) binding to rat brain membranes. In control rats a 24-hr rhythm was evident with a trough at 1000-1200 and a nadir at 0000. During the last 26 hr of a 49 hr SD period, trough and peak values were delayed by 4-6 hr. The 24-hr mean binding was significantly (P less than 0.001) different from that of controls. If sleep deprivation was followed by recovery sleep (RS), the normal rhythm of 5-HT binding was obtained already within 1 hr after SD. The effects of SD and RS were ascertained by plasma ACTH and corticosterone assay. No significant change in the hormone rhythms were observed through the mean plasma level of ACTH and corticosterone were enhanced to about 180 and 150%, respectively. Chronic treatment with the antidepressant imipramine resulted in a decrease of the 24-hr mean 5-HT binding by about 50% and a 2-hr delay of peak and trough values. Imipramine treatment decreased the peak value of 5-HT concentration at 1000 to about 65% and appears to abolish the rhythm of 5-HT concentration.
睡眠剥夺（SD）改变了大鼠脑膜中特定高亲和力血清素（5-HT）结合的昼夜节律。在对照大鼠中，24小时节律明显，在1000 - 1200时出现低谷，在0000时出现最低点。在49小时睡眠剥夺期的最后26小时内，低谷和峰值延迟了4 - 6小时。24小时平均结合率与对照组相比有显著差异（P小于0.001）。如果睡眠剥夺后进行恢复睡眠（RS），5-HT结合的正常节律在睡眠剥夺后1小时内即可恢复。通过血浆促肾上腺皮质激素（ACTH）和皮质酮测定来确定睡眠剥夺和恢复睡眠的影响。虽然ACTH和皮质酮的平均血浆水平分别提高到约180%和150%，但未观察到激素节律有显著变化。用抗抑郁药丙咪嗪进行慢性治疗导致24小时平均5-HT结合率降低约50%，峰值和谷值延迟2小时。丙咪嗪治疗使1000时5-HT浓度的峰值降至约65%，似乎消除了5-HT浓度的节律。
