Rowe M, Rowe D T, Gregory C D, Young L S, Farrell P J, Rupani H, Rickinson A B
Department of Cancer Studies, University of Birmingham, U.K.
EMBO J. 1987 Sep;6(9):2743-51. doi: 10.1002/j.1460-2075.1987.tb02568.x.
Recently established Epstein-Barr virus (EBV)-positive Burkitt's lymphoma (BL) cell lines, carrying chromosomal translocations indicative of their malignant origin, have been monitored for their degree of in vitro progression towards a more 'lymphoblastoid' cell surface phenotype and growth pattern, and for their expression of three EBV latent gene products which are constitutively present in all virus-transformed normal lymphoblastoid cell lines (LCLs). BL cell lines which stably retained the original tumour biopsy phenotype on serial passage were all positive for the nuclear antigen EBNA 1 but did not express detectable amounts of two other 'transforming' proteins, EBNA 2 and the latent membrane protein (LMP). This novel pattern of EBV gene expression was also observed on direct analysis of BL biopsy tissue. All three viral proteins became detectable, however, in BL cell lines which had progressed towards a more LCL-like phenotype in vitro. This work establishes a link between B cell phenotype and the accompanying pattern of EBV latent gene expression, and identifies a novel type of EBV:cell interaction which may be unique to BL cells.
最近建立的携带表明其恶性起源的染色体易位的爱泼斯坦 - 巴尔病毒（EBV）阳性伯基特淋巴瘤（BL）细胞系，已被监测其在体外向更“淋巴母细胞样”细胞表面表型和生长模式进展的程度，以及它们对三种EBV潜伏基因产物的表达，这三种产物在所有病毒转化的正常淋巴母细胞系（LCLs）中持续存在。在连续传代时稳定保留原始肿瘤活检表型的BL细胞系对核抗原EBNA 1均呈阳性，但不表达可检测量的其他两种“转化”蛋白EBNA 2和潜伏膜蛋白（LMP）。在对BL活检组织的直接分析中也观察到这种EBV基因表达的新模式。然而，在体外已向更类似LCL表型进展的BL细胞系中，所有三种病毒蛋白都变得可检测到。这项工作建立了B细胞表型与伴随的EBV潜伏基因表达模式之间的联系，并确定了一种可能是BL细胞特有的新型EBV：细胞相互作用。
