阴沟肠杆菌 CF-S27 利用羟胺高效去除好氧和异养氮。
Aerobic and heterotrophic nitrogen removal by Enterobacter cloacae CF-S27 with efficient utilization of hydroxylamine.
机构信息
Environmental Microbiology, Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Odisha, India.
Environmental Microbiology, Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Odisha, India.
出版信息
Bioresour Technol. 2017 May;232:285-296. doi: 10.1016/j.biortech.2017.02.049. Epub 2017 Feb 15.
Heterotrophic bacterium, Enterobacter cloacae CF-S27 exhibited simultaneous nitrification and aerobic denitrification in presence of high concentration of hydroxylamine. With the initial nitrogen concentration of 100mgLh, ammonium, nitrate and nitrite removal efficiencies were 81%, 99.9% and 92.8%, while the corresponding maximum removal rates reached as high as 11.6, 15.1 and 11.2mgLh respectively. Quantitative amplification by real time PCR and enzyme assay demonstrated that hydroxylamine reductase gene (hao) is actively involved in hetrotrophic nitrification and aerobic denitrification process of Enterobacter cloacae CF-S27. PCR primers were designed targeting amplification of hao gene from diversified environmental soil DNA. The strain Enterobacter cloacae CF-S27 significantly maintained the undetectable amount of dissolved nitrogen throughout 60days of zero water exchange fish culture experiment in domestic wastewater.
异养菌阴沟肠杆菌 CF-S27 在高浓度羟胺存在下表现出同时硝化和好氧反硝化。在初始氮浓度为 100mg/L 的条件下，铵、硝酸盐和亚硝酸盐的去除效率分别为 81%、99.9%和 92.8%，而相应的最大去除速率分别高达 11.6、15.1 和 11.2mg/L/h。实时 PCR 和酶测定定量扩增表明，羟胺还原酶基因 (hao) 积极参与阴沟肠杆菌 CF-S27 的异养硝化和好氧反硝化过程。设计了针对从多样化环境土壤 DNA 扩增 hao 基因的 PCR 引物。在家庭废水中进行的 60 天零水交换鱼类养殖实验中，阴沟肠杆菌 CF-S27 菌株显著保持了溶解氮的不可检测量。
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