Kim Kyu Yeun, Kim Do Young, Seo Jimyung, Ahn Yuri, Kim Dong Soo
Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea.
Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea.
J Korean Med Sci. 2017 Apr;32(4):599-604. doi: 10.3346/jkms.2017.32.4.599.
Quadrivalent human papillomavirus (HPV) vaccine has been reported to be significantly associated with Behçet's disease (BD). However, no reports have described HPV infection as a possible cause for the development of BD. The objective of this study was to evaluate whether anti-HPV immunoglobulin G (IgG) antibody titer is increased in BD. Serum samples from 93 Korean BD patients, who fulfilled the diagnostic criteria of the International Study Group for BD, were used in an enzyme-linked immunosorbent assay (ELISA). The clinical activity of BD was evaluated at the time of blood sampling. HPV-16 L1 virus-like particle (VLP) antigen was used in this study for the ELISA. Patients with BD had significantly higher antibody titers against HPV-16 (optical density [OD], 0.210-3.675; mean 0.992) than that of healthy controls (OD, 0.248-0.762; mean 0.517; P < 0.001). Using a receiver operating characteristic (ROC) analysis, a cut-off value of 0.578 OD for the anti-HPV antibody titer was determined that differentiated BD patients from healthy controls. When we compared the clinical features of BD between the 2 groups, articular involvement of BD was more likely in patients with an anti-HPV-16 antibody titer < 0.578 OD (P = 0.035). In addition, patients with an anti-HPV-16 antibody titer < 0.578 were significantly younger than those with a titer ≥ 0.578 OD. HPV itself may be a possible extrinsic triggering infectious agent causing the development of BD.
据报道，四价人乳头瘤病毒（HPV）疫苗与白塞病（BD）显著相关。然而，尚无报告将HPV感染描述为BD发病的可能原因。本研究的目的是评估BD患者的抗HPV免疫球蛋白G（IgG）抗体滴度是否升高。采用酶联免疫吸附测定（ELISA）检测了93例符合国际白塞病研究组诊断标准的韩国BD患者的血清样本。在采血时评估BD的临床活动度。本研究采用HPV-16 L1病毒样颗粒（VLP）抗原进行ELISA检测。BD患者针对HPV-16的抗体滴度（光密度[OD]，0.210 - 3.675；平均值0.992）显著高于健康对照（OD，0.248 - 0.762；平均值0.517；P < 0.001）。通过受试者工作特征（ROC）分析，确定抗HPV抗体滴度的截断值为0.578 OD，以此区分BD患者和健康对照。当我们比较两组BD的临床特征时，抗HPV-16抗体滴度< 0.578 OD的患者更易出现BD的关节受累（P = 0.035）。此外，抗HPV-16抗体滴度< 0.578的患者明显比滴度≥ 0.578 OD的患者年轻。HPV本身可能是导致BD发病的一种可能的外部触发感染因子。
