Szabó József, Vucskits András Valentin, Berta Erzsébet, Andrásofszky Emese, Bersényi András, Hullár István
Department of Animal Breeding, Nutrition and Laboratory Animal Science, University of Veterinary Medicine , P. O. Box 2, H-1400 Budapest , Hungary.
Europharmavet Ltd., , Budapest , Hungary.
Acta Vet Hung. 2017 Mar;65(1):66-80. doi: 10.1556/004.2017.007.
The objective of this study was to investigate the effects of fulvic acid (FA) and humic acid (HA) as the two main compounds of humic substances, separately on Fe and Mn homeostasis. Seventy-two male Wistar rats were randomly divided into 9 experimental groups. The control diet (AIN-93G formula) and diets supplemented with 0.1%, 0.2%, 0.4% and 0.8% HA or FA were fed for 26 days. Fe and Mn concentrations of the large intestinal content, liver, kidney, femur and hair were determined. No significant differences were observed in the production parameters. The effects of FA and HA on iron homeostasis were significantly different. FA proved to be a good iron source, and slightly increased the iron content of liver and kidney, but - up to a dietary iron level of 52.7 mg/kg - it did not influence the efficiency of iron absorption. Above a dietary iron level of 52.7 mg/kg down-regulation of Fe absorption can be assumed. HA significantly stimulated the iron uptake and there was no down-regulation of Fe absorption up to 0.8% dietary HA supplementation level (61.5 mg Fe/kg diet). In the HA groups the iron content of the liver and kidney decreased significantly, suggesting that in spite of the better Fe absorption, the HA-Fe complex does not provide iron to the investigated organs. Neither FA nor HA supplementation influenced the Fe content of the femur and hair and slightly decreased the Mn concentration in the large intestinal content. This effect was significant (with a 22.7% Mn concentration decrease) only at the HA supplementation rate of 0.8%. Neither FA nor HA influenced significantly the Mn concentrations of the liver, kidney and femur. The Mn concentration of the hair in rats receiving FA- or HA-supplemented diets was higher than in the control rats; however, this result needs further confirmation.
本研究的目的是分别研究腐殖质的两种主要成分富里酸（FA）和腐殖酸（HA）对铁和锰稳态的影响。将72只雄性Wistar大鼠随机分为9个实验组。给予对照饮食（AIN - 93G配方）以及添加0.1%、0.2%、0.4%和0.8% HA或FA的饮食，持续26天。测定大肠内容物、肝脏、肾脏、股骨和毛发中的铁和锰浓度。在生产参数方面未观察到显著差异。FA和HA对铁稳态的影响显著不同。FA被证明是一种良好的铁源，略微增加了肝脏和肾脏中的铁含量，但在饮食铁水平达到52.7 mg/kg之前，它不影响铁的吸收效率。在饮食铁水平高于52.7 mg/kg时，可以推测铁吸收会下调。HA显著刺激铁的摄取，在饮食中HA补充水平达到0.8%（61.5 mg铁/kg饮食）之前，铁吸收没有下调。在HA组中，肝脏和肾脏中的铁含量显著降低，这表明尽管铁吸收更好，但HA - 铁复合物并未向所研究的器官提供铁。补充FA和HA均未影响股骨和毛发中的铁含量，且略微降低了大肠内容物中的锰浓度。仅在HA补充率为0.8%时，这种影响才显著（锰浓度降低22.7%）。补充FA或HA均未显著影响肝脏、肾脏和股骨中的锰浓度。接受补充FA或HA饮食的大鼠毛发中的锰浓度高于对照大鼠；然而，这一结果需要进一步证实。
