Jiang Zhenfeng, Yao Lifen, Ma Hongge, Xu Panpan, Li Zhiyan, Guo Mian, Chen Jianhang, Bao Hongbo, Qiao Shupei, Zhao Yufang, Shen Jia, Zhu Minwei, Meyers Carolyn, Ma Guizhen, Xie Chuncheng, Liu Li, Wang Haiyang, Zhang Wang, Dong Qi, Shen Hong, Lin Zhiguo
Oncol Res. 2017 Jul 5;25(6):1009-1019. doi: 10.3727/096504016X14813859905646. Epub 2016 Dec 15.
Pyroptosis is a type of proinflammatory programmed cell death mediated by caspase 1 activity and occurs in several types of eukaryotic tumor cells, including gliomas. MicroRNAs (miRNAs), small endogenous noncoding RNAs, have been demonstrated to be advantageous in glioma therapy. However, the question of whether miRNAs regulate pyroptosis in glioma remains unknown. The current study found that caspase 1 expression was substantially increased in both glioma tissues and glioma cell lines, U87 and T98G, while miR-214 expression was significantly downregulated. Luciferase reporter assay recognized caspase 1 as a target gene of miR-214. These findings demonstrate that miR-214 could inhibit cell proliferation and migration through the regulation of pyroptosis intermediated by caspase 1 in glioma U87 and T98G cells and may suggest a novel therapeutic for the intervention of glioma.
细胞焦亡是一种由半胱天冬酶-1活性介导的促炎性程序性细胞死亡，发生于包括胶质瘤在内的多种真核肿瘤细胞中。微小RNA（miRNA）是一类内源性小分子非编码RNA，已被证明在胶质瘤治疗中具有优势。然而，miRNA是否调节胶质瘤中的细胞焦亡尚不清楚。目前的研究发现，半胱天冬酶-1在胶质瘤组织及胶质瘤细胞系U87和T98G中的表达均显著增加，而miR-214的表达则显著下调。荧光素酶报告基因检测证实半胱天冬酶-1是miR-214的靶基因。这些发现表明，miR-214可通过调节半胱天冬酶-1介导的细胞焦亡来抑制胶质瘤U87和T98G细胞的增殖和迁移，这可能为胶质瘤的干预提供一种新的治疗方法。
